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Aims <p>Nanotechnology’s wide-ranging applications, this study aims to investigate how silver nanoparticles (AgNPs) activate ROS signaling pathways to enhance stress resistance in rose seedlings and induce stress memory. It also examines the impact of AgNPs on root microbial communities and soil enzyme activities.</p> Methods <p>Growth and physiological experiments were conducted on rose seedlings exposed to varying concentrations (0, 10, and 100&#xa0;ppm) of AgNPs. Through transcriptomics and microbiomics analyses, we delved deeper into the molecular mechanisms underpinning AgNPs-induced stress resistance in rose seedlings, alongside alterations in root microbial communities.</p> Results <p>In contrast to the water-treated group, the application of low concentrations significantly enhanced parameters such as plant height, stem thickness, aboveground biomass dry weight, root vitality, and photosynthetic pigment content. Furthermore, under salt-alkali, drought, and waterlogging stress conditions, this treatment bolstered antioxidant enzyme activities and soluble sugar accumulation. Conversely, higher concentrations exhibited inhibitory effects on rose growth and physiological resistance. Analysis through ICP-MS revealed a predominant accumulation of silver elements in the roots, followed by an upward distribution along the stem towards the flowers and leaves. Notably, lower concentrations yielded reduced silver content in flowers and leaves as opposed to higher concentrations. The low concentration treatment yielded the highest activities of soil nitrate reductase, nitrite reductase, and dehydrogenase enzymes, registering at 0.17 U/g, 7.85 U/g, and 748.72 U/g, respectively. Additionally, it reshaped the composition and abundance of soil root microbial communities, whereas higher concentrations diminished soil microbial richness. Transcriptomic scrutiny uncovered significant enrichment in pathways like riboflavin metabolism, peroxisome function, glutathione metabolism, and cysteine and methionine metabolism. This was accompanied by an upregulation of key transcription factors, including WRKY, TIFY, and bHLH, thereby fortifying the resistance response in roses.</p> Conclusions <p>The activation of stress memory induced by AgNPs triggers a certain "immune effect" that helps enhance resilience to subsequent challenges and demonstrates the potential benefits of advances in nanoagriculture technology.</p> Graphical Abstract <p></p>

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Activation of ROS signaling molecule by AgNPs: molecular mechanisms in roses under abiotic stress, exploration of stress memory, and impact on root microbiota

  • 期刊论文

  • 发表日期：

    2025年3月24日

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  • Meng Zou,
  • Kai Yu,
  • Yu Huang,
  • Qianqian Sheng,
  • Ying Chen,
  • Hao Liu,
  • Zunling Zhu,
  • Ning Feng,
  • Dexing Ma,
  • Dou Dou

摘要

Aims

Nanotechnology’s wide-ranging applications, this study aims to investigate how silver nanoparticles (AgNPs) activate ROS signaling pathways to enhance stress resistance in rose seedlings and induce stress memory. It also examines the impact of AgNPs on root microbial communities and soil enzyme activities.

Methods

Growth and physiological experiments were conducted on rose seedlings exposed to varying concentrations (0, 10, and 100 ppm) of AgNPs. Through transcriptomics and microbiomics analyses, we delved deeper into the molecular mechanisms underpinning AgNPs-induced stress resistance in rose seedlings, alongside alterations in root microbial communities.

Results

In contrast to the water-treated group, the application of low concentrations significantly enhanced parameters such as plant height, stem thickness, aboveground biomass dry weight, root vitality, and photosynthetic pigment content. Furthermore, under salt-alkali, drought, and waterlogging stress conditions, this treatment bolstered antioxidant enzyme activities and soluble sugar accumulation. Conversely, higher concentrations exhibited inhibitory effects on rose growth and physiological resistance. Analysis through ICP-MS revealed a predominant accumulation of silver elements in the roots, followed by an upward distribution along the stem towards the flowers and leaves. Notably, lower concentrations yielded reduced silver content in flowers and leaves as opposed to higher concentrations. The low concentration treatment yielded the highest activities of soil nitrate reductase, nitrite reductase, and dehydrogenase enzymes, registering at 0.17 U/g, 7.85 U/g, and 748.72 U/g, respectively. Additionally, it reshaped the composition and abundance of soil root microbial communities, whereas higher concentrations diminished soil microbial richness. Transcriptomic scrutiny uncovered significant enrichment in pathways like riboflavin metabolism, peroxisome function, glutathione metabolism, and cysteine and methionine metabolism. This was accompanied by an upregulation of key transcription factors, including WRKY, TIFY, and bHLH, thereby fortifying the resistance response in roses.

Conclusions

The activation of stress memory induced by AgNPs triggers a certain "immune effect" that helps enhance resilience to subsequent challenges and demonstrates the potential benefits of advances in nanoagriculture technology.

Graphical Abstract