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Background and aims <p>Cadmium (Cd) is one of the most toxic elements and is recognized as a serious environmental contaminant. Species of <i>Brassica rapa</i> (<i>B. rapa</i>) play important roles in the human diet, but when cultivated in Cd-contaminated soils, humans may be exposed to Cd through the food chain. In this study, we aimed to characterize a Cd transporter in <i>B. rapa</i>. Natural resistance-associated macrophage proteins (NRAMPs)&#xa0;are pivotal transporters in various species.</p> Results and conclusion <p>BrNramp1 was identified and characterized in <i>B. rapa</i>. It is primarily expressed in roots and encodes a plasma membrane-localized protein. Functional assays in both yeast and <i>Arabidopsis thaliana</i> demonstrated that BrNramp1 has Mn and Cd transport activities. Additionally, <i>BrNramp1d</i>, with a 17-amino-acid deletion in the third transmembrane domain (TM3) of BrNramp1 lost its transport activities for Cd and Mn, indicating that TM3 plays an essential role in metal transport. These findings pave the way for future research aimed at developing safer, low-Cd vegetable cultivars.</p>

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BrNramp1 contributes to cadmium and manganese uptake in Brassica rapa

  • 期刊论文

  • 发表日期：

    2025年1月30日

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  • Lingxiao Zhang,
  • Pei-Pei Gao,
  • Xiao Sun,
  • Xinyu Liu,
  • Shidi Wang,
  • Kaiwen Zhang,
  • Xinyi Han,
  • Yuxuan Duan,
  • Yunjing Zhang,
  • Wen-Ju Liu

摘要

Background and aims

Cadmium (Cd) is one of the most toxic elements and is recognized as a serious environmental contaminant. Species of Brassica rapa (B. rapa) play important roles in the human diet, but when cultivated in Cd-contaminated soils, humans may be exposed to Cd through the food chain. In this study, we aimed to characterize a Cd transporter in B. rapa. Natural resistance-associated macrophage proteins (NRAMPs) are pivotal transporters in various species.

Results and conclusion

BrNramp1 was identified and characterized in B. rapa. It is primarily expressed in roots and encodes a plasma membrane-localized protein. Functional assays in both yeast and Arabidopsis thaliana demonstrated that BrNramp1 has Mn and Cd transport activities. Additionally, BrNramp1d, with a 17-amino-acid deletion in the third transmembrane domain (TM3) of BrNramp1 lost its transport activities for Cd and Mn, indicating that TM3 plays an essential role in metal transport. These findings pave the way for future research aimed at developing safer, low-Cd vegetable cultivars.