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<p>The <i>WUSCHEL</i> (<i>WUS</i>) transcription factor is the central organizer of shoot apical meristem (SAM) stem cells, yet its functions in woody plants remain poorly understood. This review synthesizes current knowledge of <i>WUS</i> and <i>WUS</i>-related homeobox (<i>WOX</i>) family genes in forest trees, spanning evolutionary genomics, vascular cambium regulation, somatic embryogenesis(SE), hormonal and environmental signal integration, and biotechnological applications. Comparative genomic analyses reveal “transitional” <i>WUS</i> genes in gymnosperms, illuminating the evolutionary trajectory from ancient to modern meristem regulation. In the vascular cambium unique to woody plants, <i>WOX4</i> maintains cambial cell identity and prevents ectopic xylem differentiation, while contrasting CLAVATA3/EMBRYO SURROUNDING REGION-related(CLE) peptide pathways—the <i>CLAVATA3</i>(<i>CLV3</i>)-<i>WUS</i> negative feedback in the SAM versus the TDIF-<i>PXY</i>-<i>WOX4</i> positive axis in the cambium—highlight <i>WUS/WOX</i> functional diversification across meristems. We discuss how <i>WUS</i> and derived functional peptides overcome regeneration recalcitrance, a major bottleneck in forest tree genetic transformation, and examine how cytokinin(CK)–auxin cross-talk, light, nutrient, and abiotic stress signals modulate <i>WUS/WOX</i> expression. Finally, we outline strategies for deploying CRISPR-based <i>WUS</i> regulation to improve wood properties, plant architecture, and stress resilience in forest trees. This integrative perspective positions the <i>WUS/WOX</i> family as a central nexus linking stem cell biology, environmental adaptation, and applied tree breeding.</p>

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WUSCHEL in forest trees: evolutionary organizer, vascular architect, and totipotency switch

  • 期刊论文

  • 发表日期：

    2026年5月19日

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  • Shanchen Zhong,
  • Shasha Wang,
  • Kai Cui

摘要

The WUSCHEL (WUS) transcription factor is the central organizer of shoot apical meristem (SAM) stem cells, yet its functions in woody plants remain poorly understood. This review synthesizes current knowledge of WUS and WUS-related homeobox (WOX) family genes in forest trees, spanning evolutionary genomics, vascular cambium regulation, somatic embryogenesis(SE), hormonal and environmental signal integration, and biotechnological applications. Comparative genomic analyses reveal “transitional” WUS genes in gymnosperms, illuminating the evolutionary trajectory from ancient to modern meristem regulation. In the vascular cambium unique to woody plants, WOX4 maintains cambial cell identity and prevents ectopic xylem differentiation, while contrasting CLAVATA3/EMBRYO SURROUNDING REGION-related(CLE) peptide pathways—the CLAVATA3(CLV3)-WUS negative feedback in the SAM versus the TDIF-PXY-WOX4 positive axis in the cambium—highlight WUS/WOX functional diversification across meristems. We discuss how WUS and derived functional peptides overcome regeneration recalcitrance, a major bottleneck in forest tree genetic transformation, and examine how cytokinin(CK)–auxin cross-talk, light, nutrient, and abiotic stress signals modulate WUS/WOX expression. Finally, we outline strategies for deploying CRISPR-based WUS regulation to improve wood properties, plant architecture, and stress resilience in forest trees. This integrative perspective positions the WUS/WOX family as a central nexus linking stem cell biology, environmental adaptation, and applied tree breeding.