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<p><i>Saxifraga stolonifera</i> is a Chinese traditional medicine for otitis media treatment. A few terpenoids were identified from <i>S. stolonifera</i> but the involved genes are unclear. Here, we mined a farnesyl pyrophosphate synthase gene (<i>SsFPS</i>) from the full-length transcriptome of <i>S. stolonifera</i> and confirmed it was expressed in cytoplasm. Subsequently, the enzymatic activity of SsFPS was characterized by in-vitro assays and heterologous expression in <i>E.coli</i>. Moreover, the gene expression pattern of <i>SsFPS</i> was positively correlated to which of a squalene synthase gene (<i>SsSS</i>) involved in triterpenoid biosynthesis and consistent to the accumulation pattern of total saponins in tissues. Taken together, SsFPS was characterized as an (<i>E</i>, <i>E</i>)-farnesyl pyrophosphate synthase in <i>S. stolonifera</i> and suggested to participate in sesquiterpenoid and triterpenoid biosynthesis which provides the support to explore the biosynthesis and pharmacological activity of triterpenoid in <i>Saxifraga.</i></p>

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Characterization of an (E, E)-farnesyl pyrophosphate synthase in Saxifraga stolonifera involved in triterpenoid biosynthesis

  • 期刊论文

  • 发表日期：

    2026年2月22日

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  • Chengjia Zhao,
  • Jing Han,
  • Shunbao Huang,
  • Xueping Su,
  • Li Cai,
  • Hualei Wang,
  • Jin Liang

摘要

Saxifraga stolonifera is a Chinese traditional medicine for otitis media treatment. A few terpenoids were identified from S. stolonifera but the involved genes are unclear. Here, we mined a farnesyl pyrophosphate synthase gene (SsFPS) from the full-length transcriptome of S. stolonifera and confirmed it was expressed in cytoplasm. Subsequently, the enzymatic activity of SsFPS was characterized by in-vitro assays and heterologous expression in E.coli. Moreover, the gene expression pattern of SsFPS was positively correlated to which of a squalene synthase gene (SsSS) involved in triterpenoid biosynthesis and consistent to the accumulation pattern of total saponins in tissues. Taken together, SsFPS was characterized as an (E, E)-farnesyl pyrophosphate synthase in S. stolonifera and suggested to participate in sesquiterpenoid and triterpenoid biosynthesis which provides the support to explore the biosynthesis and pharmacological activity of triterpenoid in Saxifraga.