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<p>Auxin is one of the major driving forces of plant development and requires careful regulation of transporter proteins to establish polar auxin transport. The <i>PIN-FORMED</i> (<i>PIN</i>) family plays a pivotal role in plant development by establishing auxin gradients that govern vascular patterning and organogenesis. However, the <i>PIN</i> family remains severely underexplored in <i>Setaria viridis</i>, a well-established model for C<sub>4</sub> monocots. In this study, we identified and characterized 13 <i>PIN</i> genes in the <i>S. viridis</i> genome. Phylogenetic and collinearity analyses revealed duplication events in the <i>SvPIN1</i>, <i>SvPIN5</i> and <i>SvPIN10</i> subfamilies. Structural analysis uncovered unique features, including potential pseudogenization of <i>SvPIN5a</i>. Expression profiling across five developmental stages unveiled the potential developmental roles of <i>SvPINs</i>, with <i>SvPIN1</i> and <i>SvPIN10</i> paralogues predominantly expressed in shoots and panicles, <i>SvPIN2</i> and <i>SvPIN9</i> in roots, while <i>SvPIN5b</i> showed leaf-enriched expression, suggesting potential involvement in leaf vascular development. Hormonal treatments in callus cultures revealed auxin-mediated upregulation of <i>SvPIN1b</i>, <i>SvPIN2</i>, <i>SvPIN5d</i>, <i>SvPIN8</i> and <i>SvPIN10a</i>. Our findings provide significant insights into the role of <i>PIN</i> genes in <i>S. viridis</i> and other C<sub>4</sub> monocots, establishing a foundation for future functional studies and offering potential targets for crop improvement through auxin transport manipulation.</p>

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The evolution and developmental expression profile of the PIN-FORMED family in Setaria viridis

  • 期刊论文

  • 发表日期：

    2025年12月24日

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  • João Marcos Fernandes-Esteves,
  • João Travassos-Lins,
  • Juan David Ferreira Gomes,
  • Marcio Alves-Ferreira

摘要

Auxin is one of the major driving forces of plant development and requires careful regulation of transporter proteins to establish polar auxin transport. The PIN-FORMED (PIN) family plays a pivotal role in plant development by establishing auxin gradients that govern vascular patterning and organogenesis. However, the PIN family remains severely underexplored in Setaria viridis, a well-established model for C4 monocots. In this study, we identified and characterized 13 PIN genes in the S. viridis genome. Phylogenetic and collinearity analyses revealed duplication events in the SvPIN1, SvPIN5 and SvPIN10 subfamilies. Structural analysis uncovered unique features, including potential pseudogenization of SvPIN5a. Expression profiling across five developmental stages unveiled the potential developmental roles of SvPINs, with SvPIN1 and SvPIN10 paralogues predominantly expressed in shoots and panicles, SvPIN2 and SvPIN9 in roots, while SvPIN5b showed leaf-enriched expression, suggesting potential involvement in leaf vascular development. Hormonal treatments in callus cultures revealed auxin-mediated upregulation of SvPIN1b, SvPIN2, SvPIN5d, SvPIN8 and SvPIN10a. Our findings provide significant insights into the role of PIN genes in S. viridis and other C4 monocots, establishing a foundation for future functional studies and offering potential targets for crop improvement through auxin transport manipulation.