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<p>The sensitivity of the <i>Vitis vinifera</i> L. to cold significantly affects both the yield and the quality of its fruit. Members of the WRKY transcription factor (TF) family are involved in the regulation of plant cold tolerance. In this study, we isolated <i>VaWRKY72</i> from the extremely cold-tolerant grape species <i>Vitis amurensis</i>. The transcript level of <i>VaWRKY72</i> varied among organs, but was highest in leaves. Bioinformatics analysis shows that VaWRKY72 contains a typical WRKYGQK conserved domain, a structural feature that classifies it within the Group IIb subfamily of WRKY TFs. VaWRKY72 was shown to be distributed in the nucleus and to have transcriptional activation activity in yeast. Through heterologous overexpression in <i>Arabidopsis thaliana</i> and transient transformation in <i>V. vinifera</i> L. ‘Chardonnay’, we identified that <i>VaWRKY72</i> plays a positive regulatory role in cold tolerance. This correlated with lower malondialdehyde content and electrolyte leakage, reduced accumulation of reactive oxygen species (ROS), but elevated activity of antioxidant enzymes and proline content compared with wild type. Moreover, <i>VaWRKY72</i> is revealed to act as an up-regulator of numerous genes related to cold responses in transgenic lines. The results establish a basis for understanding the mechanism of cold tolerance and so facilitate molecular breeding in grapevine.</p>

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The transcription factor VaWRKY72 from Vitis amurensis positively regulates cold tolerance in Arabidopsis thaliana and grapevine

  • 期刊论文

  • 发表日期：

    2025年9月11日

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  • Jiahui Ma,
  • Xiaoxiao Yan,
  • Xingcheng Qi,
  • Fang Ding,
  • Xinyi Hao,
  • Shijin Yang,
  • Weirong Xu,
  • Xiuming Zhang

摘要

The sensitivity of the Vitis vinifera L. to cold significantly affects both the yield and the quality of its fruit. Members of the WRKY transcription factor (TF) family are involved in the regulation of plant cold tolerance. In this study, we isolated VaWRKY72 from the extremely cold-tolerant grape species Vitis amurensis. The transcript level of VaWRKY72 varied among organs, but was highest in leaves. Bioinformatics analysis shows that VaWRKY72 contains a typical WRKYGQK conserved domain, a structural feature that classifies it within the Group IIb subfamily of WRKY TFs. VaWRKY72 was shown to be distributed in the nucleus and to have transcriptional activation activity in yeast. Through heterologous overexpression in Arabidopsis thaliana and transient transformation in V. vinifera L. ‘Chardonnay’, we identified that VaWRKY72 plays a positive regulatory role in cold tolerance. This correlated with lower malondialdehyde content and electrolyte leakage, reduced accumulation of reactive oxygen species (ROS), but elevated activity of antioxidant enzymes and proline content compared with wild type. Moreover, VaWRKY72 is revealed to act as an up-regulator of numerous genes related to cold responses in transgenic lines. The results establish a basis for understanding the mechanism of cold tolerance and so facilitate molecular breeding in grapevine.