Scholarlink

<p>The root is one of the most important organs that determines the final grain yield in rice. Auxin is essential for root development in plants. Rice auxin response factor7 (OsARF7), belonging to the ARF family, is a key regulator of root development. Here, we show that OsARF7 positively regulates root development via auxin signaling. The <i>osarf7</i> mutants display a significant decrease in the root number, adventitious root (AR) number and length, and primary root (PR) length, compared with the wild-type. Exogenous NAA treatment significantly suppresses PR length in <i>osarf7</i> mutants, <i>OsARF7-OE</i> lines, and its wild-type, does not affect the root number of <i>osarf7</i> mutants, but suppresses the biomass of <i>osarf7</i> mutants. At the molecular level, <i>OsARF7</i> is preferentially expressed in the culm, root, and leaf, especially highly expressed in the tips of the PR, AR, root pericycle, and lateral root (LR) primordia; meanwhile, <i>OsARF7</i> expression is significantly enhanced by exogenous NAA treatment, suggesting that the positive regulatory role of OsARF7 on root development is based on auxin signaling. A series of biochemical and genetic analyses demonstrate that OsARF7 functions upstream of <i>OsCRL1</i> and acts downstream of <i>OsMADS25</i> to regulate root development via auxin signaling. To conclude, OsARF7 is a key positive regulatory factor that regulates root development by activating the expression of <i>OsCRL1</i> via auxin signaling, by which, OsMADS25 positively mediates <i>OsARF7</i> expression in rice. This work provides valuable insight into the regulatory mechanism controlling root development and a genetic resource for the molecular improvement of root architecture.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

The involvement of auxin response factor OsARF7 in positively regulating root development by mediating the expression of OsCRL1 in rice (Oryza sativa L.)

  • 期刊论文

  • 发表日期：

    2025年2月26日

查看全文
  • Congying Sun,
  • Kai Fan,
  • Xin Wang,
  • Honghai Liu,
  • Nuoping Guo,
  • Wanyu Liu,
  • Guixiang Ye,
  • Weiwei Lin,
  • Wenxiong Lin,
  • Zhaowei Li

摘要

The root is one of the most important organs that determines the final grain yield in rice. Auxin is essential for root development in plants. Rice auxin response factor7 (OsARF7), belonging to the ARF family, is a key regulator of root development. Here, we show that OsARF7 positively regulates root development via auxin signaling. The osarf7 mutants display a significant decrease in the root number, adventitious root (AR) number and length, and primary root (PR) length, compared with the wild-type. Exogenous NAA treatment significantly suppresses PR length in osarf7 mutants, OsARF7-OE lines, and its wild-type, does not affect the root number of osarf7 mutants, but suppresses the biomass of osarf7 mutants. At the molecular level, OsARF7 is preferentially expressed in the culm, root, and leaf, especially highly expressed in the tips of the PR, AR, root pericycle, and lateral root (LR) primordia; meanwhile, OsARF7 expression is significantly enhanced by exogenous NAA treatment, suggesting that the positive regulatory role of OsARF7 on root development is based on auxin signaling. A series of biochemical and genetic analyses demonstrate that OsARF7 functions upstream of OsCRL1 and acts downstream of OsMADS25 to regulate root development via auxin signaling. To conclude, OsARF7 is a key positive regulatory factor that regulates root development by activating the expression of OsCRL1 via auxin signaling, by which, OsMADS25 positively mediates OsARF7 expression in rice. This work provides valuable insight into the regulatory mechanism controlling root development and a genetic resource for the molecular improvement of root architecture.