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<p>The lipoxygenase (<i>LOX</i>) gene family is widely distributed in plants, and its activity is closely associated with seed viability and stress tolerance. In this study, we cloned the rice(<i>Oryza sativa</i>)lipoxygenase gene <i>OsLOX1</i>, a key participant in the 13-lipoxygenase metabolic pathway. Our primary focus was to investigate its role in mediating responses to drought stress and seed germination in rice. Histochemical staining and qPCR analysis indicated that the expression level of <i>OsLOX1</i> was relatively high in leaves and early germinating seeds. Our findings revealed that mutant lines with CRISPR/Cas9-induced knockout of <i>OsLOX1</i> exhibited reduced tolerance to drought stress compared with the wild-type. This was accompanied by elevated levels of H<sub>2</sub>O<sub>2</sub> and malondialdehyde, and a decrease in the expression levels of genes associated with antioxidant enzymes. Furthermore, knockout of <i>OsLOX1</i> reduced the longevity of rice seeds increased H<sub>2</sub>O<sub>2</sub> and MDA levels, and decreased the activities of the antioxidant enzymes superoxide dismutase and catalase, compared with the wild-type. These findings demonstrated that <i>OsLOX1</i> positively regulated rice seed vigor and drought stress.</p>

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OsLOX1 positively regulates seed vigor and drought tolerance in rice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月14日

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  • Yahong Weng,
  • Yanwen Wang,
  • Kewu Wang,
  • Fangxi Wu,
  • Yidong Wei,
  • Jiahuang Jiang,
  • Yongsheng Zhu,
  • Fuxiang Wang,
  • Hongguang Xie,
  • Yanjia Xiao,
  • Qiuhua Cai,
  • Huaan Xie,
  • Jianfu Zhang

摘要

The lipoxygenase (LOX) gene family is widely distributed in plants, and its activity is closely associated with seed viability and stress tolerance. In this study, we cloned the rice(Oryza sativa)lipoxygenase gene OsLOX1, a key participant in the 13-lipoxygenase metabolic pathway. Our primary focus was to investigate its role in mediating responses to drought stress and seed germination in rice. Histochemical staining and qPCR analysis indicated that the expression level of OsLOX1 was relatively high in leaves and early germinating seeds. Our findings revealed that mutant lines with CRISPR/Cas9-induced knockout of OsLOX1 exhibited reduced tolerance to drought stress compared with the wild-type. This was accompanied by elevated levels of H2O2 and malondialdehyde, and a decrease in the expression levels of genes associated with antioxidant enzymes. Furthermore, knockout of OsLOX1 reduced the longevity of rice seeds increased H2O2 and MDA levels, and decreased the activities of the antioxidant enzymes superoxide dismutase and catalase, compared with the wild-type. These findings demonstrated that OsLOX1 positively regulated rice seed vigor and drought stress.