Scholarlink

Purpose <p>Adjuvants are critical for enhancing immune responses to recombinant protein-based vaccines, which typically exhibit weak immunogenicity. Microneedle array patches (MAPs) offer a promising method for intradermal delivery, but conventional Co-Delivery MAPs (containing antigen and adjuvant together) have limited loading capacity and potential undesirable interactions. Adjuvants may also trigger adverse reactions in sensitive populations. This study aimed to develop a Dual-Delivery MAP system that enables separate and sequential administration of antigens and adjuvants, addressing these limitations and supporting personalized vaccination strategies.</p> Methods <p>The Dual-Delivery MAP was developed using a coated MAP for ovalbumin (OVA) and a dissolving MAP for the CTA1 adjuvant. Patches were sequentially applied to the same skin site. Delivery efficiency, intradermal distribution, and immunogenicity were evaluated and compared to a conventional Co-Delivery MAP and an intramuscular (IM) injection group (OVA and CTA1 in solution). OVA- and CTA1-specific IgG titers were measured to assess immune responses.</p> Results <p>Both Dual-Delivery and Co-Delivery MAPs demonstrated similar delivery efficiency (~ 70%). However, the Dual-Delivery MAP elicited significantly higher IgG titers against CTA1 and OVA compared to the Co-Delivery MAP, likely due to enhanced adjuvant functionality. The Dual-Delivery MAP induced immune responses comparable to IM injection, indicating that sequential delivery preserves adjuvant activity and enhances immunogenicity.</p> Conclusions <p>The Dual-Delivery MAP represents a novel, modular approach for skin-targeted vaccination. By separating antigen and adjuvant into distinct patches, it improves stability, maintains adjuvant efficacy, and enables personalized vaccine administration, offering a promising strategy for effective and safe vaccination.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Development and Evaluation of Dual Microneedle Array Patch for Sequential Intradermal Delivery of Adjuvant and Antigen

  • 期刊论文

  • 发表日期：

    2025年9月4日

查看全文
  • Ye-Lim Lee,
  • Hye-Ran Cha,
  • Da-Eun Lee,
  • Minwoo Ryu,
  • Hyeon Woo Chung,
  • Sunghoon Park,
  • Jung-ah Choi,
  • Seung-Ki Baek,
  • Jae Myun Lee,
  • Jung-Hwan Park

摘要

Purpose

Adjuvants are critical for enhancing immune responses to recombinant protein-based vaccines, which typically exhibit weak immunogenicity. Microneedle array patches (MAPs) offer a promising method for intradermal delivery, but conventional Co-Delivery MAPs (containing antigen and adjuvant together) have limited loading capacity and potential undesirable interactions. Adjuvants may also trigger adverse reactions in sensitive populations. This study aimed to develop a Dual-Delivery MAP system that enables separate and sequential administration of antigens and adjuvants, addressing these limitations and supporting personalized vaccination strategies.

Methods

The Dual-Delivery MAP was developed using a coated MAP for ovalbumin (OVA) and a dissolving MAP for the CTA1 adjuvant. Patches were sequentially applied to the same skin site. Delivery efficiency, intradermal distribution, and immunogenicity were evaluated and compared to a conventional Co-Delivery MAP and an intramuscular (IM) injection group (OVA and CTA1 in solution). OVA- and CTA1-specific IgG titers were measured to assess immune responses.

Results

Both Dual-Delivery and Co-Delivery MAPs demonstrated similar delivery efficiency (~ 70%). However, the Dual-Delivery MAP elicited significantly higher IgG titers against CTA1 and OVA compared to the Co-Delivery MAP, likely due to enhanced adjuvant functionality. The Dual-Delivery MAP induced immune responses comparable to IM injection, indicating that sequential delivery preserves adjuvant activity and enhances immunogenicity.

Conclusions

The Dual-Delivery MAP represents a novel, modular approach for skin-targeted vaccination. By separating antigen and adjuvant into distinct patches, it improves stability, maintains adjuvant efficacy, and enables personalized vaccine administration, offering a promising strategy for effective and safe vaccination.