Objective <p>To develop and apply noninvasive analytical technologies to detect falsified vaccines and drugs.</p> Methods <p>Univariate and multivariate methods, based on the water nuclear magnetic resonance (<i>w</i>NMR) technology, was explored as a tool for noninvasive detection of simple and sophisticated falsified vaccines, biotherapeutics and anesthetics. All <i>w</i>NMR measurements were done noninvasively on sealed vials, prefilled syringes, and injection pens. Saline USP served as a simple falsified drug product while highly similar vaccines and drugs (brand vs. generics/biosimilars) served as each other’s sophisticated falsified drug products for technology development and testing.</p> Results <p>Simple falsified drug products, represented by USP saline, can be differentiated from authentic vaccines and drugs by univariate <i>w</i>NMR. Sophisticated falsified drug products, represented by highly similar products, can be differentiated from authentic ones by univariate <i>w</i>NMR in some cases and by multivariate <i>w</i>NMR in all cases, attesting to the high discriminating power of <i>w</i>NMR in product authentication. The multivariate methods generate 2D and 3D data arrays that may serve as fingerprints of vaccines and drugs.</p> Conclusions <p>A suite of noninvasive analytical methods, based on the <i>w</i>NMR technology, have been developed to detect falsified vaccines and drugs. <i>w</i>NMR analyzes vaccines and drugs in their primary containers without opening and uses benchtop instruments with no wet lab operations and no reagents. <i>w</i>NMR detection of&#xa0;falsified drug products may be implemented in low-resource settings for enhanced pharmaceutical security and preventive pharmacovigilance.</p>