<p>HPLC techniques for quantitative determination of myricitrin (myricetin-3-<i>O</i>-α-L-rhamnopyranoside) in extracts from leaves of common myrtle (<i>Myrtus communis</i> L.) were developed. The contents of myricitrin in tincture, infusion, and thick extract of myrtle leaves were determined as 0.68 ± 0.02; 0.17 ± 0.01 and 12.75 ± 0.05%, respectively. The error in a single determination of the myricitrin content in tincture, infusion, and thick extract of <i>Myrtus communis</i> L. leaves with 95% confidence probability was ±4.61; ±5.87, and ±2.33%, respectively. The expediency of standardization of the extracts from <i>Myrtus communis</i> L. leaves in terms of the content of the dominant and diagnostically significant flavonoid myricitrin using the HPLC technique and detection at a wavelength of 360 nm was substantiated.</p>

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HPLC Determination of Myricitrin Content in Extracts From Leaves of Myrtus communis L.

  • V. D. Maslova,
  • V. A. Kurkin

摘要

HPLC techniques for quantitative determination of myricitrin (myricetin-3-O-α-L-rhamnopyranoside) in extracts from leaves of common myrtle (Myrtus communis L.) were developed. The contents of myricitrin in tincture, infusion, and thick extract of myrtle leaves were determined as 0.68 ± 0.02; 0.17 ± 0.01 and 12.75 ± 0.05%, respectively. The error in a single determination of the myricitrin content in tincture, infusion, and thick extract of Myrtus communis L. leaves with 95% confidence probability was ±4.61; ±5.87, and ±2.33%, respectively. The expediency of standardization of the extracts from Myrtus communis L. leaves in terms of the content of the dominant and diagnostically significant flavonoid myricitrin using the HPLC technique and detection at a wavelength of 360 nm was substantiated.