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<p>Polycystic ovary syndrome (PCOS) is a multifaceted endocrine-metabolic disorder that not only leads to reproductive complications but also adversely affects other systems of the body. Ovarian fibrosis is considered to be one of the pathological mechanisms of PCOS. The purpose of this research was to explore whether traditional Chinese medicine monomers kaempferol can improve the epithelial-mesenchymal transition of ovarian stromal cells and the mechanism of its effect. The ovarian stromal cell fibrosis model was induced by cisplatin and divided into four groups. Immunofluorescence technology was applied to identify ovarian stromal cells, molecular docking method was applied to screen potential effective monomers, CCK8 method was used to detect cell viability in each group,Western Blot and RT-PCR were used to detect the expression of fibrosis-associated factors in the cell model, and TGF - β1/Smad3 pathway-related factors were experimentally verified. Compared with the control group, the protein expression levels of α-SMA, TGF- β1, TGFBR1, Smad3, and p-Smad3 in the cisplatin group were significantly increased (P &lt; 0.05), and the mRNA levels of α-SMA, TGF- β1, and ALK5 were significantly increased (P &lt; 0.05). Compared with the cisplatin group, the protein expression of α-SMA, TGF- β, TGFBR1, Smad3, and p-Smad3 in the low-dose and high-dose kaempferol groups was markedly decreased (P &lt; 0.05), and the mRNAlevels of α-SMA, TGF-β1, and ALK5 was noticeably decreased (P &lt; 0.05). Kaempferol may inhibit the epithelial-mesenchymal transition of ovarian stromal cells by regulating the TGF- β1/Smad3 signaling pathway.</p>

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Kaempferol Suppresses the Epithelial-to-Mesenchymal Transition of Ovarian Stromal Sells Via the TGF-β1/Smad3 Signaling

  • 期刊论文

  • 发表日期：

    2026年1月1日

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  • Senfeng Liu,
  • Lili Lin,
  • Yuehong Chen,
  • Honglin Dong,
  • Shuyuan Xie,
  • Yuanjie Qiu

摘要

Polycystic ovary syndrome (PCOS) is a multifaceted endocrine-metabolic disorder that not only leads to reproductive complications but also adversely affects other systems of the body. Ovarian fibrosis is considered to be one of the pathological mechanisms of PCOS. The purpose of this research was to explore whether traditional Chinese medicine monomers kaempferol can improve the epithelial-mesenchymal transition of ovarian stromal cells and the mechanism of its effect. The ovarian stromal cell fibrosis model was induced by cisplatin and divided into four groups. Immunofluorescence technology was applied to identify ovarian stromal cells, molecular docking method was applied to screen potential effective monomers, CCK8 method was used to detect cell viability in each group,Western Blot and RT-PCR were used to detect the expression of fibrosis-associated factors in the cell model, and TGF - β1/Smad3 pathway-related factors were experimentally verified. Compared with the control group, the protein expression levels of α-SMA, TGF- β1, TGFBR1, Smad3, and p-Smad3 in the cisplatin group were significantly increased (P < 0.05), and the mRNA levels of α-SMA, TGF- β1, and ALK5 were significantly increased (P < 0.05). Compared with the cisplatin group, the protein expression of α-SMA, TGF- β, TGFBR1, Smad3, and p-Smad3 in the low-dose and high-dose kaempferol groups was markedly decreased (P < 0.05), and the mRNAlevels of α-SMA, TGF-β1, and ALK5 was noticeably decreased (P < 0.05). Kaempferol may inhibit the epithelial-mesenchymal transition of ovarian stromal cells by regulating the TGF- β1/Smad3 signaling pathway.