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<p>A method for quantitative determination of rutin content in Japanese sophora (<i>Sophora japonica</i> L.) buds by reversed-phase HPLC in isocratic mode on a Milichrom-6 microcolumn liquid chromatograph using a reference standard of pharmacopoeial rutin (3-<i>O</i>-rutinoside quercetin) has been developed. The rutin content in Japanese sophora buds was determined to vary from 10.96 ± 0.40 to 15.20 ± 0.51%. The error of a single determination of the rutin content in Japanese sophora buds was ±6.49% with a confidence probability of 95%.</p>

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HPLC Determination of Rutin Content in Japanese Sophora Buds

  • 期刊论文

  • 发表日期：

    2025年8月1日

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  • V. A. Kurkin,
  • M. K. Cherednik

摘要

A method for quantitative determination of rutin content in Japanese sophora (Sophora japonica L.) buds by reversed-phase HPLC in isocratic mode on a Milichrom-6 microcolumn liquid chromatograph using a reference standard of pharmacopoeial rutin (3-O-rutinoside quercetin) has been developed. The rutin content in Japanese sophora buds was determined to vary from 10.96 ± 0.40 to 15.20 ± 0.51%. The error of a single determination of the rutin content in Japanese sophora buds was ±6.49% with a confidence probability of 95%.