Scholarlink

<p>A fluorescent probe (<i>N</i>-(4-methyl-2-oxo-2<i>H</i>-chromen-7-yl)-2,4-dinitrobenzenesulfonamide) for selective detection of tiopronin was synthesized and characterized. The probe displays weak fluorescence attributed to electron transfer from the fluorophore to the electron-withdrawing 2,4-dinitrobenzenesulfonyl group. However, upon effective cleavage of the electron-withdrawing 2,4-dinitrobenzenesulfonyl moiety by tiopronin from the weakly fluorescent probe, the fluorescence intensity is significantly enhanced. Under optimal conditions, the fluorescence intensity exhibited a linear correlation with tiopronin concentration within the range of 30 nM to 50 μM. The proposed strategy is characterized by its simplicity, specificity, and sensitivity. The proposed probe was employed for the determination of tiopronin in urine samples and commercial tablets, demonstrating potential applications in disease diagnosis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A Selective Fluorescent Probe for Quantitative Detection of Tiopronin Based on Fluorescence Enhancement

  • 期刊论文

  • 发表日期：

    2025年5月1日

查看全文
  • Yongfen Bao,
  • Xingfeng Wang,
  • Minjuan Wang,
  • Shigang Shan,
  • Mingjie Wei,
  • Yingbao Ou

摘要

A fluorescent probe (N-(4-methyl-2-oxo-2H-chromen-7-yl)-2,4-dinitrobenzenesulfonamide) for selective detection of tiopronin was synthesized and characterized. The probe displays weak fluorescence attributed to electron transfer from the fluorophore to the electron-withdrawing 2,4-dinitrobenzenesulfonyl group. However, upon effective cleavage of the electron-withdrawing 2,4-dinitrobenzenesulfonyl moiety by tiopronin from the weakly fluorescent probe, the fluorescence intensity is significantly enhanced. Under optimal conditions, the fluorescence intensity exhibited a linear correlation with tiopronin concentration within the range of 30 nM to 50 μM. The proposed strategy is characterized by its simplicity, specificity, and sensitivity. The proposed probe was employed for the determination of tiopronin in urine samples and commercial tablets, demonstrating potential applications in disease diagnosis.