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<p>Anaplastic thyroid cancer (ATC) is one of the most common endocrine malignancies. This cancer is a rare and deadly form of thyroid cancer. Despite conventional chemotherapy, a considerable number of patients show a developing chemoresistance to it. Therefore, there is a necessity to find novel therapeutic approaches for treating anaplastic thyroid cancer patients. The aim of this project was to assess <i>in vitro</i> the cytotoxicity efficacy of phytosynthesized selenium nanoparticles using <i>Polylophium involucratum</i> (Pall.) Boiss. “<i>P. involucratum</i>” seed extract on 8305C cell lines. The phytosynthesis of selenium nanoparticles (Se-NPs) was here characterized by UV-Vis, FT-IR, XRD, DLS, Zeta, EDX, TEM and FE-SEM. The results of the phytochemical screening of hydroalcoholic extract of the seeds of <i>P. involucratum</i> included the presence of terpenoids, flavonoids, phenols, saponins, coumarins, cardiac glycosides, tannins, and quinones. The cytotoxicity activity of the synthesized (Se-NPs) and hydroalcoholic extract of seeds <i>P. involucratum</i> was tested using MTT assay. This cytotoxicity is dose dependent, and the results of cell cytotoxicity Se-NPs and extract on 8305C cell lines at 20 and 30 μg/ml concentrations showed that the IC<sub>50</sub> values were 50.63 ± 0. 3, and 50.12 ± 0.02 μg/ml, respectively. Our project suggests that Se-NPs using <i>P. involucratum</i> seed extract could be used as a therapeutic agent in anaplastic thyroid cancer. Further laboratory and animal studies are needed and recommended to evaluate the exact molecular and clinical properties of Se-NPs using <i>P. involucratum</i> seed extract.</p>

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A Novel Assessment of In Vitro Cytotoxicity Efficacy of Phytosynthesized Selenium Nanoparticles Using Polylophium involucratum (Pall.) Boiss. Seeds Extract on 8305C Cell Lines

  • 期刊论文

  • 发表日期：

    2025年2月1日

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  • Shahab Ojani,
  • Naser Montazeri,
  • Masoud Mohammadi Zeydi,
  • Masoud Ghane

摘要

Anaplastic thyroid cancer (ATC) is one of the most common endocrine malignancies. This cancer is a rare and deadly form of thyroid cancer. Despite conventional chemotherapy, a considerable number of patients show a developing chemoresistance to it. Therefore, there is a necessity to find novel therapeutic approaches for treating anaplastic thyroid cancer patients. The aim of this project was to assess in vitro the cytotoxicity efficacy of phytosynthesized selenium nanoparticles using Polylophium involucratum (Pall.) Boiss. “P. involucratum” seed extract on 8305C cell lines. The phytosynthesis of selenium nanoparticles (Se-NPs) was here characterized by UV-Vis, FT-IR, XRD, DLS, Zeta, EDX, TEM and FE-SEM. The results of the phytochemical screening of hydroalcoholic extract of the seeds of P. involucratum included the presence of terpenoids, flavonoids, phenols, saponins, coumarins, cardiac glycosides, tannins, and quinones. The cytotoxicity activity of the synthesized (Se-NPs) and hydroalcoholic extract of seeds P. involucratum was tested using MTT assay. This cytotoxicity is dose dependent, and the results of cell cytotoxicity Se-NPs and extract on 8305C cell lines at 20 and 30 μg/ml concentrations showed that the IC50 values were 50.63 ± 0. 3, and 50.12 ± 0.02 μg/ml, respectively. Our project suggests that Se-NPs using P. involucratum seed extract could be used as a therapeutic agent in anaplastic thyroid cancer. Further laboratory and animal studies are needed and recommended to evaluate the exact molecular and clinical properties of Se-NPs using P. involucratum seed extract.