Assay of Salicin Conent in White Poplar Populus alba L. Leaves by HPLC

Isoquercitrin, isorhamnetin 3-O-β-D-glucopyranoside, salicin, and 6′-O-caffeoyl-salicin, a new natural compound, were isolated from white poplar (Populus alba L.) leaves by column chromatography. An HPLC assay of the dominant phenolic glycoside, salicin, in white poplar leaves was developed. The salicin content in white poplar leaves varied from 3.19 ± 0.08% to 7.73 ± 0.01%. The error of a single determination of salicin content in white poplar leaves with a confidence probability of 95% was ± 5.51%.