Radiocomplexation, Quality Control and Bioevaluation of [^99mTc]tricarbonyl Rolipram for Brain Imaging in Mice

In this work, the complex of [^99mTc]tricarbonyl rolipram was labeled utilizing a [^99mTc]tricarbonyl core. Many optimal parameters have been used such as substrate (100 μg), pH of the reaction mixture (pH 9), reaction time (30 min), as well as temperature (100°C), providing an optimal radiochemical purity of 97.0%. Biodistribution investigations of our radiotracer, [^99mTc]tricarbonyl rolipram, were conducted on normal Swiss Albino mice. The results indicated maximum brain uptakes of 8.2 %ID/g tissue at 10 min post injection (p.i.), which cleared from the brain with time until it reached 1.90 ± 0.17% at 1 h p.i. Therefore, [^99mTc]tricarbonyl rolipram complex may be an excessively bio-selective receptor-tracer for brain ulcer imaging through the phosphodiesterase-4 inhibitor.