Improved and Validated Quantitative Spectroscopic Analysis of Chlorpheniramine in Pharmaceutical Formulations

The aim of our study is to develop a simple, delicate, precise and economical protocol for the qualitative and quantitative determination of Chlorpheniramine using spectrophotometer. Our validation is distinctive and provides a precise picture of a very weak chromophore of chlorpheniramine (CPN) that had not been accurately detected before by traditional techniques. The blue color which formed by mixing pheniramine and Cobalt- thiocyanate solution was extracted with chloroform. CPN presented a linearity range of 25 – 125 μg at 628 nm. Limit of quantification (15 μg/mL) and limit of detection (08 μg/mL) of cobalt-chloride were analyzed. The resulting data was statistically significant. Because of the appropriate reproducibility and reliability of this proposed analysis, it can be used to screen CPN in pharmaceutical preparations. It is also validated through the International Council of Harmonization for accuracy, linearity, precision, LOD and LOQ. Various dosage forms of pheniramine were evaluated and have shown that they did not exceed their relative standard deviation by 1%. Their percentage purity was within 99.62 – 103.34%. The suspected sample was treated with 2 – 3 drops of cyanocobalamine after which violet coloration appeared. The percentage relative standard deviation of accuracy and precision was acquired as 1.57 and 1.15, respectively, exactly following the authentication criteria for newly established methods. Because of the profound potential of these developed methods, including precise, fast, flawless and inexpensive, it can easily be adopted in laboratories for the routine analysis of Chlorpheniramine.