Peptide Composition and Identification of a Peptide with Anti-Inflammatory and Antiplatelet Properties in Cattle Prostate Extract
摘要
An attempt to identify the peptide structure of bovine prostate extract using water/vinegar technology followed by tangential ultrafiltration is described. Protein-peptide components with known functional activity were revealed. A new previously unknown peptide, a fragment of bovine chemokine MCP-1, was selected from the discovered regulatory peptide fragments, synthesized, and tested in three biological assays. The ability of the peptide to suppress the in vitro migration of monocytic cells and platelet aggregation was demonstrated. The newly identified peptide fragment was evaluated as promising for developing a synthetic analog of prostate extract to be used in the treatment of diseases of autoimmune and pseudo-allergenic nature.