<p>Cold atmospheric plasma (CAP) finds numerous applications across various sectors, including industry (e.g. surface modification) and medicine (e.g. tissue regeneration, wound healing, oncology, and dentistry). However, understanding the mechanical properties of materials undergoing CAP treatment is of great importance, particularly for applications involving changes in material properties. This study aims to utilize CAP as an excitation device for assessing the mechanical properties of a polymethyl methacrylate (PMMA) sample. CAP was employed to induce vibrations on a PMMA sample around its resonance frequency, and the resulting vibrations were measured by a scanning laser doppler vibrometer. The elastic modulus of the PMMA sample was then calculated based on the stress and strain profiles obtained from the measured vibrations. The obtained elastic modulus value of 4.87 GPa showed excellent agreement with the 4.83 GPa value obtained using other excitation devices, indicating the reliability of CAP in mechanical characterization. This study is the first step toward potential applications that can break new ground in the use of CAP in monitoring and characterization of mechanical properties during CAP treatment (e.g. surface treatment), paving the way for enhanced control and optimization of CAP-based processes in various applications.</p> Graphical Abstract <p></p>