<p>We study theoretically the analytical expression of a pulsed chirped modified anomalous vortex beam (MAVB) across biological tissue using the integral of Huygens-Fresnel and the Fourier Transform method. Spectral modifications for pulsed chirped MAVB are examined across various biological tissues, including human upper dermis, mouse intestinal epithelium, and mouse deep dermis. Graphical representations are employed to analyze the beam parameters, biological tissue effect properties, and transverse positions on the analyzed beam. Results reveal that the structure constant of refractive index, chirp parameter, pulse duration, and variations in beam parameters can impact the intensity of the spectral beam. The on-axis spectral intensity shows a blue shift, whereas the off-axis spectral intensity demonstrates a red shift as the radial coordinates increase. It is expected that the results of this study will greatly enhance progress in disease and treatment, particularly in cancer research. By analyzing variations in intensity distribution, scientists can improve their ability to identify and diagnose diseases more effectively.</p>