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<p>Alzheimer’s disease (AD) is a chronic neurodegenerative disorder characterized by damage to neural structures in different areas of the brain, including loss of synapses. The causes of destructive changes and neuronal death are poorly understood. The mechanisms of synapse loss are unclear. During the development of AD, structural and functional changes occur in synapses, and these are interconnected with the morphology of postsynaptic formations, i.e., dendritic spines. This paper describes a technology for chronic visualization of dendritic spines in transgenic animals using multiphoton fluorescence microscopy. Hybrid animals (5xFAD-M) expressing green fluorescent protein and a model of AD were used. The methodological solutions presented here provide a means of identifying the dynamics of dendritic spine density over periods of several days and tracking the transformation of their morphological types over long observation periods.</p>

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Chronic Imaging of Dendritic Spine Morphology in Transgenic Mice of the 5xFAD-M Hybrid Strain, a Model of Alzheimer’s Disease

  • 期刊论文

  • 发表日期：

    2025年3月1日

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  • A. E. Matukhno,
  • P. V. Tkacheva,
  • V. B. Voinov,
  • L. V. Lysenko,
  • E. V. Evsyukova,
  • A. O. Taisaeva,
  • I. B. Bezprozvanny

摘要

Alzheimer’s disease (AD) is a chronic neurodegenerative disorder characterized by damage to neural structures in different areas of the brain, including loss of synapses. The causes of destructive changes and neuronal death are poorly understood. The mechanisms of synapse loss are unclear. During the development of AD, structural and functional changes occur in synapses, and these are interconnected with the morphology of postsynaptic formations, i.e., dendritic spines. This paper describes a technology for chronic visualization of dendritic spines in transgenic animals using multiphoton fluorescence microscopy. Hybrid animals (5xFAD-M) expressing green fluorescent protein and a model of AD were used. The methodological solutions presented here provide a means of identifying the dynamics of dendritic spine density over periods of several days and tracking the transformation of their morphological types over long observation periods.