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<p>The accurate and rapid detection of <i>Mycobacterium tuberculosis</i> (<i>M</i>. <i>tuberculosis</i>) is essential for the effective treatment of tuberculosis. In this work, perovskite/silica nanocomposites CsPbBr<sub>3</sub>@MSNs-PbBrOH (DP-CPB) were synthesized via a dual in situ-coating strategy, encapsulating CsPbBr<sub>3</sub> nanocrystals with mesoporous silica and PbBrOH. The resulting nanocomposites exhibited excellent stability in physiological media. The nanocomposites’ surface was conjugated with the MS10-Trunc aptamer specific for <i>Mtb</i> malate synthase (<i>MtbMS</i>). Leveraging the aptamer’s high affinity, we developed two fluorescent aptasensors: one based on a 96-well plate for <i>MtbMS</i> detection, and another employing magnetic nanoparticles for the detection of <i>Mtb</i> bacterial strains (<i>Mtb</i> H37Rv). The sensors demonstrate dynamic ranges of 50-750&#xa0;nM for <i>MtbMS</i> and 10<sup>2</sup>-10⁷ CFU/mL for <i>Mtb</i> H37Rv, with low limits of detection (LOD) of 1.17&#xa0;nM and 3&#xa0;CFU/mL, respectively. The aptasensors possess the comprehensive advantages of the highly efficient photoluminescence of DP-CPB, high specificity, and fast detection of <i>MtbMS</i> and H37Rv. The aptasensor was successfully applied for the determination of <i>Mtb</i> H37Rv, revealing the vast potential of perovskites in biosensing.</p>

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Highly stable perovskite nanocrystal based fluorescent aptasensor for sensitive detection of MtbMS and Mtb H37Rv

  • 期刊论文

  • 发表日期：

    2026年1月27日

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  • Yuchen Wu,
  • Yuting Zhong,
  • Yanhong Niu,
  • Zhili Wu,
  • Lingbing Liao,
  • Qianfang Hu,
  • Xiaolan Yang

摘要

The accurate and rapid detection of Mycobacterium tuberculosis (M. tuberculosis) is essential for the effective treatment of tuberculosis. In this work, perovskite/silica nanocomposites CsPbBr3@MSNs-PbBrOH (DP-CPB) were synthesized via a dual in situ-coating strategy, encapsulating CsPbBr3 nanocrystals with mesoporous silica and PbBrOH. The resulting nanocomposites exhibited excellent stability in physiological media. The nanocomposites’ surface was conjugated with the MS10-Trunc aptamer specific for Mtb malate synthase (MtbMS). Leveraging the aptamer’s high affinity, we developed two fluorescent aptasensors: one based on a 96-well plate for MtbMS detection, and another employing magnetic nanoparticles for the detection of Mtb bacterial strains (Mtb H37Rv). The sensors demonstrate dynamic ranges of 50-750 nM for MtbMS and 102-10⁷ CFU/mL for Mtb H37Rv, with low limits of detection (LOD) of 1.17 nM and 3 CFU/mL, respectively. The aptasensors possess the comprehensive advantages of the highly efficient photoluminescence of DP-CPB, high specificity, and fast detection of MtbMS and H37Rv. The aptasensor was successfully applied for the determination of Mtb H37Rv, revealing the vast potential of perovskites in biosensing.