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<p>The thermal stability of core–shell quantum dots (QDs) encapsulated apoferritin has been sparingly reported. In this study, we reported the spontaneous encapsulation of mercaptopropionic acid functionalized CdSe:CdS:ZnS core–shell QDs and temperature-induced structural changes of QDs-apoferritin composite using biophysical techniques and negative stain single particle analysis. An increase in absorbance and decrease in tryptophan fluorescence intensity was observed by increasing QDs concentration (0–250&#xa0;ng/mL). A change in circular dichroism characteristic peaks was observed with increasing temperatures (25&#xa0;°C, 37&#xa0;°C, and 55&#xa0;°C). HR-TEM image also showed an increase in size (12.0 ± 1.0&#xa0;nm at 25&#xa0;°C, 12.5 ± 1.0&#xa0;nm at 37&#xa0;°C, and 15 ± 1.3&#xa0;nm at 55&#xa0;°C) along with 6 ± 1% and 68 ± 5% release of QDs (than 25&#xa0;°C) from the composite at 37&#xa0;°C and 55&#xa0;°C, respectively. The single particle analysis confirmed the encapsulation of four QD particles at 25&#xa0;°C but showed multiple 2D class averages at 37&#xa0;°C, confirming the destabilization and molten globule-like structure at 55&#xa0;°C. This study revealed that QDs induced significant structural alteration in the apoferritin at a much lower temperature than its normal melting temperature (80&#xa0;°C).</p>

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Molecular interaction and temperature-induced structural alteration of hydrophilic CdSe:CdS:ZnS quantum dots-apoferritin composite

  • 期刊论文

  • 发表日期：

    2025年1月22日

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  • Shikha Chaudhary,
  • Anjali Maurya,
  • Uddipan Das,
  • Ravi Mani Tripathi,
  • Subhash Chandra Yadav

摘要

The thermal stability of core–shell quantum dots (QDs) encapsulated apoferritin has been sparingly reported. In this study, we reported the spontaneous encapsulation of mercaptopropionic acid functionalized CdSe:CdS:ZnS core–shell QDs and temperature-induced structural changes of QDs-apoferritin composite using biophysical techniques and negative stain single particle analysis. An increase in absorbance and decrease in tryptophan fluorescence intensity was observed by increasing QDs concentration (0–250 ng/mL). A change in circular dichroism characteristic peaks was observed with increasing temperatures (25 °C, 37 °C, and 55 °C). HR-TEM image also showed an increase in size (12.0 ± 1.0 nm at 25 °C, 12.5 ± 1.0 nm at 37 °C, and 15 ± 1.3 nm at 55 °C) along with 6 ± 1% and 68 ± 5% release of QDs (than 25 °C) from the composite at 37 °C and 55 °C, respectively. The single particle analysis confirmed the encapsulation of four QD particles at 25 °C but showed multiple 2D class averages at 37 °C, confirming the destabilization and molten globule-like structure at 55 °C. This study revealed that QDs induced significant structural alteration in the apoferritin at a much lower temperature than its normal melting temperature (80 °C).