Detection of ciprofloxacin in ear drop using UV spectrophotometric method based on gold nanoparticles as a sensing probe

Ciprofloxacin is a fluoroquinolone group antibiotic used to treat variety of bacterial illnesses. The purpose of this study is to develop a rapid and accurate spectrophotometric approach for the detection of ciprofloxacin using gold nanoparticles (GNPs). Localized surface plasmon resonance (LSPR) absorption band of GNPs at 523.0 nm was utilized as the spectrophotometric detection of ciprofloxacin. To determine the optimum GNPs concentration and pH value of the phosphate buffer solution used in the experimental research, the effect of pH values and GNPs concentrations in the absorbance value of ciprofloxacin-GNPs was investigated. The linear operating range for the developed spectrophotometric method was 0.015–1.48 µg/mL in pH 7.0 phosphate buffer solution. In the recovery study conducted to detect the amount of ciprofloxacin in commercial ear drops, the recovery value was calculated as 91.06 ± 1.21%.