<p>Researchers have been studying self-healing in concrete for many years as a potential solution for self-repairing concrete structures. Bacterial concrete is one of the concrete types with self-healing characteristics. However, introducing and maintaining the required environment for bacteria is a challenging task. This study used biochar from the agricultural and food waste industries as an immobilizing agent for self-healing concrete containing Bacillus subtilis bacteria. As the self-healing due to bacteria is an alkaliphilic reaction, three cement types were used to investigate the self-healing characteristics. Three different methods, which included compressive strength recovery (M1), damage cycles using ultrasonic pulse velocity (M2), and the semicircular bending (SCB) test (M3), were used to quantify self-healing potential. In M1, control and bacterial concrete were loaded to a certain damage level, and self-healing was quantified based on strength recovery up to 84 days, while in M2, UPV was measured until the specimens were found to be intact. In M3, self-healing was quantified for concrete specimens subjected to tensile load. The M1 method indicated that ordinary Portland cement (OPC) exhibited on average 56.52% self-healing due to bacterial activity, while Portland Pozzolana and slag cement exhibited 20.82% and 49.67%, respectively. Further, the M2 method indicated that the degree of recovery in bacterial concrete was better than that of the control concrete. In addition, the M3 method, which is a first-of-its-kind test to quantify self-healing, showed that bacterial concrete was able to sustain a higher number of loading cycles compared to control specimens. The statistical analysis also indicated a significant effect of treatment and cement type on the self-healing potential.</p>