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Background <p>X-chromosome short tandem repeats (X-STRs) are valuable for resolving complex kinship cases. Most population databases are based on the ArgusX12 kit (Qiagen), using PCR and capillary electrophoresis (CE) to identify length-based (LB) alleles, and are formatted for FamlinkX software. However, haplotype diversity is often underrepresented by population studies, compelling database updates (<a href="https://famlink.se/fx_databases.html">https://famlink.se/fx_databases.html</a>). The ForenSeq DNA Signature Prep kit (Verogen) uses massive parallel sequencing (MPS) to analyze 7 X-STRs and report sequence-based (SB) alleles, for which population databases are currently unavailable. Because these 7 markers are a subset of the ArgusX12 panel, existing LB-based databases can support kinship interpretation of MPS-derived data.</p> Methods and results <p>We therefore updated a Mexican population database for 12 X-STRs and generated a corresponding 7-marker subset with FamlinkX-compatible files. Using 500 haplotypes from the Investigator Argus X-12 kit QS (Qiagen), we estimated forensic parameters and evaluated the impact of the updated database by calculating likelihood ratios (LRs) in four representative kinship scenarios. The updated database (<i>n</i> = 1433) increased LRs by an average of 55% compared to the previous version (<i>n</i> = 933).</p> Conclusions <p>These results support improved forensic interpretation in Mexican and Latin American populations lacking comprehensive national X-STR databases based on ArgusX12 and ForenSeq DNA Signature kits.</p>

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Update of Mexican population databases for complex kinship interpretation with twelve and seven X-STRs

  • 期刊论文

  • 发表日期：

    2026年6月27日

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  • Irán Cortés-Trujillo,
  • Mayra Elizabeth García-Aceves,
  • Eduardo Rojas-Prado,
  • Mauro López-Armenta,
  • Cinthya Miroslava Guzmán-González,
  • Guadalupe Avalos-Navarro,
  • Gabriela Martínez-Cortés,
  • José Alonso Aguilar-Velázquez,
  • Mariano Guardado-Estrada,
  • Héctor Rangel-Villalobos

摘要

Background

X-chromosome short tandem repeats (X-STRs) are valuable for resolving complex kinship cases. Most population databases are based on the ArgusX12 kit (Qiagen), using PCR and capillary electrophoresis (CE) to identify length-based (LB) alleles, and are formatted for FamlinkX software. However, haplotype diversity is often underrepresented by population studies, compelling database updates (https://famlink.se/fx_databases.html). The ForenSeq DNA Signature Prep kit (Verogen) uses massive parallel sequencing (MPS) to analyze 7 X-STRs and report sequence-based (SB) alleles, for which population databases are currently unavailable. Because these 7 markers are a subset of the ArgusX12 panel, existing LB-based databases can support kinship interpretation of MPS-derived data.

Methods and results

We therefore updated a Mexican population database for 12 X-STRs and generated a corresponding 7-marker subset with FamlinkX-compatible files. Using 500 haplotypes from the Investigator Argus X-12 kit QS (Qiagen), we estimated forensic parameters and evaluated the impact of the updated database by calculating likelihood ratios (LRs) in four representative kinship scenarios. The updated database (n = 1433) increased LRs by an average of 55% compared to the previous version (n = 933).

Conclusions

These results support improved forensic interpretation in Mexican and Latin American populations lacking comprehensive national X-STR databases based on ArgusX12 and ForenSeq DNA Signature kits.