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Background <p>Greengram (<i>Vigna radiata</i>) productivity is constrained by major pests and diseases, and introgression of ricebean (<i>Vigna umbellata)</i> derived genomic segments conferring disease resistance through interspecific hybridization offers an effective strategy to broaden the genetic base and enhance resistance to biotic stresses.</p> Methods and Results <p>In the present study, three advanced pre-breeding lines (COGRISC 1, COGRISC 2 and COGRISC 3) developed through interspecific crosses between greengram (CO 6 and CO 7) and ricebean (RBL 35 and LRB 576), were evaluated. These lines exhibited ricebean-like qualitative traits, including hypogeal germination and purple hypocotyl colour, while biometrical traits showed intermediate expression between the parents. The pre-breeding lines exhibited partial fertility, ranged from 37.50 to 78.50%. To confirm ricebean genome introgression, 155 adzuki bean–derived SSR markers were screened for parental polymorphism, of which 124 (80%) were polymorphic and used for genotyping. Ricebean-specific marker alleles predominated in the pre-breeding lines, with 71 (57.30%) markers detected in COGRISC 1 and COGRISC 2 and 65 (52.40%) markers in COGRISC 3. Graphical genotype analysis revealed a mosaic pattern of introgression across all linkage groups with predominance of ricebean genomic segments. Cluster analysis grouped ricebean parents and pre-breeding lines together. All lines exhibited disease-free reactions against Mungbean Yellow Mosaic Virus (MYMV), powdery mildew, and leaf crinkle disease under artificial screening, and to cercospora leaf spot under field conditions.</p> Conclusions <p>The results demonstrate that ricebean-derived genomic segments confer multiple disease resistance, highlighting the potential of these lines as donor material for greengram improvement.</p>

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Genome analysis and introgression of multiple disease resistance into pre-breeding lines derived from greengram × ricebean crosses

  • 期刊论文

  • 发表日期：

    2026年6月30日

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  • P. Venkatesh,
  • K. Anandhi,
  • L. Karthiba,
  • P. Renukadevi,
  • S. Vellaikumar,
  • T. Nivethitha,
  • P. Jayamani

摘要

Background

Greengram (Vigna radiata) productivity is constrained by major pests and diseases, and introgression of ricebean (Vigna umbellata) derived genomic segments conferring disease resistance through interspecific hybridization offers an effective strategy to broaden the genetic base and enhance resistance to biotic stresses.

Methods and Results

In the present study, three advanced pre-breeding lines (COGRISC 1, COGRISC 2 and COGRISC 3) developed through interspecific crosses between greengram (CO 6 and CO 7) and ricebean (RBL 35 and LRB 576), were evaluated. These lines exhibited ricebean-like qualitative traits, including hypogeal germination and purple hypocotyl colour, while biometrical traits showed intermediate expression between the parents. The pre-breeding lines exhibited partial fertility, ranged from 37.50 to 78.50%. To confirm ricebean genome introgression, 155 adzuki bean–derived SSR markers were screened for parental polymorphism, of which 124 (80%) were polymorphic and used for genotyping. Ricebean-specific marker alleles predominated in the pre-breeding lines, with 71 (57.30%) markers detected in COGRISC 1 and COGRISC 2 and 65 (52.40%) markers in COGRISC 3. Graphical genotype analysis revealed a mosaic pattern of introgression across all linkage groups with predominance of ricebean genomic segments. Cluster analysis grouped ricebean parents and pre-breeding lines together. All lines exhibited disease-free reactions against Mungbean Yellow Mosaic Virus (MYMV), powdery mildew, and leaf crinkle disease under artificial screening, and to cercospora leaf spot under field conditions.

Conclusions

The results demonstrate that ricebean-derived genomic segments confer multiple disease resistance, highlighting the potential of these lines as donor material for greengram improvement.