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Background <p>A<b> s</b>light, transient increase in heat shock induces stage-specific detrimental effect on reproductive performance of riverine buffaloes (<i>Bubalus bubalis</i>). The vulnerability of oocytes and early embryos, as well as their ability to recover are both restricted and stage-dependent, consequently form major basis for reproduction failure during heat stress.</p> Objective <p>The current study aimed to identify the most sensitive and susceptible stage of oocytes and early embryos against short thermal stress which hampers the reproduction significantly, and intent to unravel the underlying molecular signals responsible for the poor cellular growth and stunted embryonic development.</p> Material and Method <p>Six different in vitro experimental groups of oocytes and early embryos were exposed to 40ºC for 4&#xa0;h during different stages of in vitro maturation (IVM) and <i>in-vitro</i> fertilization (IVF), as well as in vitro culture (IVC) of early embryos to assess the stage dependent effect of heat stress on buffalo oocytes and early embryos.</p> Result <p>The most detrimental effect of heat stress was observed in group 2 with sharp reduction in morula to blastocysts transition rate (<i>P</i> &lt; 0.05). The underlying molecular signals of <i>HSP8</i>, <i>MnSOD</i> and <i>SIRT3</i> genes were significantly increased from mid-IVM oocytes to 4-cell embryo. Disruption of molecular signal network of genes viz; <i>eIF1A</i>, <i>U2AF</i>, <i>BAX</i>, <i>BCL2</i>, <i>CASPASE9</i>, <i>TEAD4</i>, <i>CDX2</i>, <i>ZO-1</i>, <i>ETS2</i> and <i>NKB1</i> was implicated in cellular damage, inconsistent and embryonic development, consequently leading to formation of poor quality of blastocysts with reduced inner cell mass (ICM) and trophectoderm (TE) cells in the affected groups.</p> Conclusion <p>The current study provides insight into the molecular and cellular effects of short thermal stress on buffaloes oocytes and early embryos, demonstrating development potential and identifying buffalo oocytes and pre-morulae embryos as the most vulnerable stages.</p>

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Transient heat stress irreversibly disrupts molecular signals, cellular and developmental competence of riverine buffaloes (Bubalus bubalis) oocytes and early embryos in a stage-specific manner

  • 期刊论文

  • 发表日期：

    2026年3月26日

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  • Devender Singh,
  • Parul Sarwalia,
  • Manish Kumar,
  • Moloya Gohain,
  • Asit Jain,
  • Tripti Jain,
  • Rakesh Kumar,
  • Tirtha Kumar Datta

摘要

Background

A slight, transient increase in heat shock induces stage-specific detrimental effect on reproductive performance of riverine buffaloes (Bubalus bubalis). The vulnerability of oocytes and early embryos, as well as their ability to recover are both restricted and stage-dependent, consequently form major basis for reproduction failure during heat stress.

Objective

The current study aimed to identify the most sensitive and susceptible stage of oocytes and early embryos against short thermal stress which hampers the reproduction significantly, and intent to unravel the underlying molecular signals responsible for the poor cellular growth and stunted embryonic development.

Material and Method

Six different in vitro experimental groups of oocytes and early embryos were exposed to 40ºC for 4 h during different stages of in vitro maturation (IVM) and in-vitro fertilization (IVF), as well as in vitro culture (IVC) of early embryos to assess the stage dependent effect of heat stress on buffalo oocytes and early embryos.

Result

The most detrimental effect of heat stress was observed in group 2 with sharp reduction in morula to blastocysts transition rate (P < 0.05). The underlying molecular signals of HSP8, MnSOD and SIRT3 genes were significantly increased from mid-IVM oocytes to 4-cell embryo. Disruption of molecular signal network of genes viz; eIF1A, U2AF, BAX, BCL2, CASPASE9, TEAD4, CDX2, ZO-1, ETS2 and NKB1 was implicated in cellular damage, inconsistent and embryonic development, consequently leading to formation of poor quality of blastocysts with reduced inner cell mass (ICM) and trophectoderm (TE) cells in the affected groups.

Conclusion

The current study provides insight into the molecular and cellular effects of short thermal stress on buffaloes oocytes and early embryos, demonstrating development potential and identifying buffalo oocytes and pre-morulae embryos as the most vulnerable stages.