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Background <p>Parkinson’s disease (PD) is the second most common neurodegenerative disease globally, characterized by the abnormal aggregation of α-synuclein. Recent studies suggest that circular RNAs (circRNAs), predominantly formed by back-splicing of pre-mRNAs, and the proteins they encode are implicated in neurodegenerative diseases; however, the specific role of circular RNA-encoded proteins in PD is understudied. This study investigated the expression and functional role of the circFKBP8(5S,6)-encoded protein (cFKBP8), translated from the circular RNA circFKBP8(5S,6), in a cellular model of PD.</p> Methods and Results <p>An in vitro PD model was created by treating SH-SY5Y cells with 1-methyl-4-phenylpyridinium (MPP⁺). cFKBP8 expression was assessed using immunofluorescence staining and quantitative real-time polymerase chain reaction (RT-qPCR). An increase in cFKBP8 expression was found in MPP⁺-treated cells compared to untreated controls. Functional analyses using Western blot revealed that cFKBP8 overexpression significantly increased α-synuclein expression. In contrast, silencing cFKBP8 decreased α-synuclein levels in SH-SY5Y cells.</p> Conclusions <p>These results indicate that cFKBP8 expression is increased in a cellular model of PD and can influence α-synuclein expression in SH-SY5Y cells, thereby highlighting the role of circRNA-encoded proteins in α-synuclein biology, with potential implications for developing biomarkers and therapeutic strategies for PD.</p>

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circFKBP8(5S,6)-encoded protein modulates α-synuclein expression in SH-SY5Y cells

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月2日

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  • Jude Uzoechina,
  • Zhijun Zhang

摘要

Background

Parkinson’s disease (PD) is the second most common neurodegenerative disease globally, characterized by the abnormal aggregation of α-synuclein. Recent studies suggest that circular RNAs (circRNAs), predominantly formed by back-splicing of pre-mRNAs, and the proteins they encode are implicated in neurodegenerative diseases; however, the specific role of circular RNA-encoded proteins in PD is understudied. This study investigated the expression and functional role of the circFKBP8(5S,6)-encoded protein (cFKBP8), translated from the circular RNA circFKBP8(5S,6), in a cellular model of PD.

Methods and Results

An in vitro PD model was created by treating SH-SY5Y cells with 1-methyl-4-phenylpyridinium (MPP⁺). cFKBP8 expression was assessed using immunofluorescence staining and quantitative real-time polymerase chain reaction (RT-qPCR). An increase in cFKBP8 expression was found in MPP⁺-treated cells compared to untreated controls. Functional analyses using Western blot revealed that cFKBP8 overexpression significantly increased α-synuclein expression. In contrast, silencing cFKBP8 decreased α-synuclein levels in SH-SY5Y cells.

Conclusions

These results indicate that cFKBP8 expression is increased in a cellular model of PD and can influence α-synuclein expression in SH-SY5Y cells, thereby highlighting the role of circRNA-encoded proteins in α-synuclein biology, with potential implications for developing biomarkers and therapeutic strategies for PD.