Scholarlink

Background <p>Rice productivity is severely challenged by the brown planthopper (<i>Nilaparvata lugens</i> Stål, BPH) infestations. Four different biotypes and multiple virulent BPH populations make the pest control even more challenging. Among these biotypes, the biotype 4 found in the Indian sub-continent is considered to be the most destructive. However, the molecular mechanisms of the BPH biotype 4 and rice interactions are explored to a limited extent.</p> Methods <p>In this study, the function of a carboxylesterase gene (<i>NleFE4</i>) in the BPH biotype 4 has been investigated via RNA intereference-mediated gene silencing during compatible (BPH fed on TN1 rice) and incompatible (BPH fed on PTB33 rice) rice-BPH interactions. In addition, the <i>NleFE4</i> silencing effects on the BPH performance and survival were evaluated. Finally, interactions between NleFE4 and key rice secondary metabolites (sakuranetin, phenylpropane, epigallocatechin, spermidine, and quercetin) were predicted.</p> Results <p>Administration of ds<i>NleFE4</i> significantly reduced the <i>NleFE4</i> expression in the BPH. The honeydew amount, BPH weight gain, and BPH survival assays suggested that silencing of <i>NleFE4</i> negatively affected the BPH performance and survival. The molecular docking analysis results suggested that NleFE4 could interact with all five rice secondary metabolites with varied levels of binding affinities and could be involved in the detoxification of these metabolites during rice-BPH interactions.</p> Conclusion <p>Silencing of <i>NleFE4</i> had detrimental effects on the BPH performance and survival. NleFE4 could be involved in the detoxification of key rice allelochemicals.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Silencing of a carboxylesterase gene NleFE4 in Indian brown planthopper biotype negatively impacts its performance and survival on rice

  • 期刊论文

  • 发表日期：

    2026年3月16日

查看全文
  • Nihal Ravindranath,
  • Swapna Rani Nag,
  • Ashutosh Nayak,
  • Dinakar Gaikwad,
  • Satya Narayan Sahu,
  • Satyabrata Nanda

摘要

Background

Rice productivity is severely challenged by the brown planthopper (Nilaparvata lugens Stål, BPH) infestations. Four different biotypes and multiple virulent BPH populations make the pest control even more challenging. Among these biotypes, the biotype 4 found in the Indian sub-continent is considered to be the most destructive. However, the molecular mechanisms of the BPH biotype 4 and rice interactions are explored to a limited extent.

Methods

In this study, the function of a carboxylesterase gene (NleFE4) in the BPH biotype 4 has been investigated via RNA intereference-mediated gene silencing during compatible (BPH fed on TN1 rice) and incompatible (BPH fed on PTB33 rice) rice-BPH interactions. In addition, the NleFE4 silencing effects on the BPH performance and survival were evaluated. Finally, interactions between NleFE4 and key rice secondary metabolites (sakuranetin, phenylpropane, epigallocatechin, spermidine, and quercetin) were predicted.

Results

Administration of dsNleFE4 significantly reduced the NleFE4 expression in the BPH. The honeydew amount, BPH weight gain, and BPH survival assays suggested that silencing of NleFE4 negatively affected the BPH performance and survival. The molecular docking analysis results suggested that NleFE4 could interact with all five rice secondary metabolites with varied levels of binding affinities and could be involved in the detoxification of these metabolites during rice-BPH interactions.

Conclusion

Silencing of NleFE4 had detrimental effects on the BPH performance and survival. NleFE4 could be involved in the detoxification of key rice allelochemicals.