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Background <p>Lily (<i>Lilium brownii</i>), a perennial bulbous herbaceous plant in the <i>Lilium</i> genus of the Liliaceae family, is esteemed in traditional Chinese medicine and utilized as a functional food due to the high nutritional content of its bulbs. However, the cultivation of lilies is severely constrained by viral infections, which can cause substantial yield losses and markedly compromise both commercial production and medicinal value. Virus disease has become a significant threat to lily production worldwide and has occurred in several provinces of China.</p> Methods and results <p>In this study, a new isolate of apple stem grooving virus (ASGV-LS) (<i>Capillovirus mali</i>) from <i>L. brownii</i> in Lishui City, Zhejiang province, China was first identified using the next-generation sequencing (NGS) technique combined with reverse transcription PCR and 3′/5′-rapid amplification of cDNA ends methods. ASGV-LS possessed a positive-sense RNA genome with a full-length sequence of 6469 nucleotides (nt), including a 27-nt poly(A) tail and two overlapping open reading frames (ORFs). ORF1 encoded a 241.0-kDa polyprotein, whereas ORF2 encoded a 36.1-kDa movement protein. Sequence analysis revealed that ASGV-LS had the highest nucleotide sequence identity of 94.5% with ASGV-HZ (GenBank: MW259059) isolated from <i>Gynura japonica</i>. Further phylogenetic analysis of the complete genome sequences indicated that ASGV-LS clusters into a subclade with ASGV-HZ, ASGV-PG (GenBank: MK427058), ASGV-ZG2 (GenBank: MN495979), and ASGV-KFP (GenBank: KR106996), which belong to the genus <i>Capillovirus</i>.</p> Conclusion <p>These findings establish ASGV-LS is a new isolate of the ASGV species of the genus <i>Capillovirus</i> in the family <i>Betaflexiviridae</i>. To our knowledge, this is the first report of <i>L. brownii</i> being infected by ASGV in China.</p>

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First report of apple stem grooving virus infecting a species of Lilium (Lilium brownii) in China

  • 期刊论文

  • 发表日期：

    2026年2月24日

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  • Yang Shen,
  • Qundan Lv,
  • Guokai Xia,
  • Fang Ye,
  • Zhoufo Lu,
  • Zhanqi Wang,
  • Xueting Zhong

摘要

Background

Lily (Lilium brownii), a perennial bulbous herbaceous plant in the Lilium genus of the Liliaceae family, is esteemed in traditional Chinese medicine and utilized as a functional food due to the high nutritional content of its bulbs. However, the cultivation of lilies is severely constrained by viral infections, which can cause substantial yield losses and markedly compromise both commercial production and medicinal value. Virus disease has become a significant threat to lily production worldwide and has occurred in several provinces of China.

Methods and results

In this study, a new isolate of apple stem grooving virus (ASGV-LS) (Capillovirus mali) from L. brownii in Lishui City, Zhejiang province, China was first identified using the next-generation sequencing (NGS) technique combined with reverse transcription PCR and 3′/5′-rapid amplification of cDNA ends methods. ASGV-LS possessed a positive-sense RNA genome with a full-length sequence of 6469 nucleotides (nt), including a 27-nt poly(A) tail and two overlapping open reading frames (ORFs). ORF1 encoded a 241.0-kDa polyprotein, whereas ORF2 encoded a 36.1-kDa movement protein. Sequence analysis revealed that ASGV-LS had the highest nucleotide sequence identity of 94.5% with ASGV-HZ (GenBank: MW259059) isolated from Gynura japonica. Further phylogenetic analysis of the complete genome sequences indicated that ASGV-LS clusters into a subclade with ASGV-HZ, ASGV-PG (GenBank: MK427058), ASGV-ZG2 (GenBank: MN495979), and ASGV-KFP (GenBank: KR106996), which belong to the genus Capillovirus.

Conclusion

These findings establish ASGV-LS is a new isolate of the ASGV species of the genus Capillovirus in the family Betaflexiviridae. To our knowledge, this is the first report of L. brownii being infected by ASGV in China.