Scholarlink

Background <p>Molecular characterization of local <i>Pseudomonas aeruginosa</i> (<i>P. aeruginosa</i>) isolates offers valuable insights into strain clonality, resistance mechanisms, and virulence factors linked to nosocomial infections. This study aims to examine resistance patterns, the prevalence of four clinically relevant metallo-β-lactamase (MBL) gene families, virulence gene profiles, and the genetic diversity among local isolates.</p> Methods <p>Between March 2022 and March 2024, clinical isolates of <i>P. aeruginosa</i> were collected and identified using standard phenotypic methods, with confirmation achieved through targeting the gyrB gene. Antimicrobial susceptibility testing was conducted via the disc diffusion method. PCR assays were performed to detect carbapenemase genes (<i>bla</i><sub>VIM</sub>, <i>bla</i><sub>NDM1</sub>, <i>bla</i><sub>IMP</sub>, <i>bla</i><sub>SPM</sub>, <i>bla</i><sub>KPC</sub>, <i>bla</i><sub>OXA-48</sub>, and <i>bla</i><sub>OXA-181</sub>) and virulence genes. Genotyping of <i>P. aeruginosa</i> was executed using random amplified polymorphic DNA (RAPD)-PCR analysis.</p> Results <p>A total of 154 unique clinical isolates of <i>P. aeruginosa</i> were collected from various specimen types, primarily from respiratory and wound samples. Resistance rates were alarmingly high for all tested antimicrobial agents, exceeding 76% for most antibiotics. The frequencies of the <i>bla</i><sub>IMP</sub> and <i>bla</i><sub>NDM1</sub> genes were found to be 59.7% and 10.4%, respectively. Additionally, the frequencies of the <i>toxA</i>, <i>exoU</i>, and <i>exoS</i> genes were 97.4%, 78.6%, and 18.8%, respectively. RAPD-PCR analysis revealed twelve distinct clusters.</p> Conclusion <p>The high prevalence of multidrug-resistant <i>P. aeruginosa</i>, along with the widespread presence of metallo-beta-lactamase (MBL) and virulence genes, highlights the risk of severe clinical outcomes in hospital-acquired infections. Molecular typing reveals genetic diversity and clonal relationships, underscoring the importance of continuous molecular surveillance to inform effective infection control measures and targeted treatment strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Genetic diversity, detection of virulence genes and high prevalence of blaIMP metallo-β-lactamases (MBLs) in clinical isolates of Pseudomonas aeruginosa in Bandar Abbas, Southern Iran

  • 期刊论文

  • 发表日期：

    2026年3月3日

查看全文
  • Saeed Shoja,
  • Maryam Cheshmberah,
  • Jebreil Shamseddin,
  • Fatemeh Hajizadeh,
  • Abbas Farahani

摘要

Background

Molecular characterization of local Pseudomonas aeruginosa (P. aeruginosa) isolates offers valuable insights into strain clonality, resistance mechanisms, and virulence factors linked to nosocomial infections. This study aims to examine resistance patterns, the prevalence of four clinically relevant metallo-β-lactamase (MBL) gene families, virulence gene profiles, and the genetic diversity among local isolates.

Methods

Between March 2022 and March 2024, clinical isolates of P. aeruginosa were collected and identified using standard phenotypic methods, with confirmation achieved through targeting the gyrB gene. Antimicrobial susceptibility testing was conducted via the disc diffusion method. PCR assays were performed to detect carbapenemase genes (blaVIM, blaNDM1, blaIMP, blaSPM, blaKPC, blaOXA-48, and blaOXA-181) and virulence genes. Genotyping of P. aeruginosa was executed using random amplified polymorphic DNA (RAPD)-PCR analysis.

Results

A total of 154 unique clinical isolates of P. aeruginosa were collected from various specimen types, primarily from respiratory and wound samples. Resistance rates were alarmingly high for all tested antimicrobial agents, exceeding 76% for most antibiotics. The frequencies of the blaIMP and blaNDM1 genes were found to be 59.7% and 10.4%, respectively. Additionally, the frequencies of the toxA, exoU, and exoS genes were 97.4%, 78.6%, and 18.8%, respectively. RAPD-PCR analysis revealed twelve distinct clusters.

Conclusion

The high prevalence of multidrug-resistant P. aeruginosa, along with the widespread presence of metallo-beta-lactamase (MBL) and virulence genes, highlights the risk of severe clinical outcomes in hospital-acquired infections. Molecular typing reveals genetic diversity and clonal relationships, underscoring the importance of continuous molecular surveillance to inform effective infection control measures and targeted treatment strategies.