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Background <p>Soil contamination with Cadmium (Cd) is one of the major environmental issues facing the world, which poses a tremendous threat to plant growth. Heavy-metal ATPase (HMA), essential to regulate plants’ metal homeostasis, has been extensively identified in abundant plant species. However, the HMA gene family members in <i>Chrysanthemum indicum</i> have not been identified.</p> Methods and results <p>In this study, we identified 10 <i>CiHMAs</i> and classified them into three subfamilies: I, II, and III. Transcriptome and qRT-PCR analyses demonstrated significant induction of <i>CiHMA5</i> expression under Cd stress. <i>CiHMA5</i> is situated in the chloroplast and cell membrane. Heterologous expression of <i>CiHMA5</i> in <i>Arabidopsis thaliana</i> exhibits a Cd-tolerant phenotype with diminished Cd accumulation as well as enhanced antioxidant enzyme activity. Furthermore, <i>CiHMA5</i> transgenic <i>C. indicum</i> plants also conferred a Cd-tolerant phenotype and lower Cd content, accompanied by higher transfer coefficients in shoots and leaves than wild-type (WT).</p> Conlusion <p>: These findings established a theoretical groundwork for further delve into the role and regulatory mechanism of <i>CiHMAs</i> in <i>Chrysanthemum</i> under Cd stress.</p>

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Genome-wide analysis of HMA gene family in Chrysanthemum indicum and positive role of CiHMA5 in cd tolerance

  • 期刊论文

  • 发表日期：

    2026年1月28日

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  • Kaiyuan Zhang,
  • Chuyi Chen,
  • Shuguang Liu,
  • Shengyan Chen,
  • Xingyu Ni,
  • Liran Yue,
  • Miao He

摘要

Background

Soil contamination with Cadmium (Cd) is one of the major environmental issues facing the world, which poses a tremendous threat to plant growth. Heavy-metal ATPase (HMA), essential to regulate plants’ metal homeostasis, has been extensively identified in abundant plant species. However, the HMA gene family members in Chrysanthemum indicum have not been identified.

Methods and results

In this study, we identified 10 CiHMAs and classified them into three subfamilies: I, II, and III. Transcriptome and qRT-PCR analyses demonstrated significant induction of CiHMA5 expression under Cd stress. CiHMA5 is situated in the chloroplast and cell membrane. Heterologous expression of CiHMA5 in Arabidopsis thaliana exhibits a Cd-tolerant phenotype with diminished Cd accumulation as well as enhanced antioxidant enzyme activity. Furthermore, CiHMA5 transgenic C. indicum plants also conferred a Cd-tolerant phenotype and lower Cd content, accompanied by higher transfer coefficients in shoots and leaves than wild-type (WT).

Conlusion

: These findings established a theoretical groundwork for further delve into the role and regulatory mechanism of CiHMAs in Chrysanthemum under Cd stress.