Background <p>Histone acetylation is essential for histone-to-protamine transition during spermiogenesis. JADE1 contributes to the assembly of the JADE1-HBO1 complex and facilitates HBO1-mediated histone acetylation. However, whether the JADE1-HBO1 complex regulates histone acetylation in spermiogenesis is unknown.</p> Methods and results <p>In this study, we generated a germ cell-specific <i>Jade1</i> conditional knockout mouse (<i>Jade1</i> cKO) and analysed the spermatogenesis and fertility. Despite <i>Jade1</i> cKO males exhibiting reduced testes weight, histological analysis and nuclear surface spreading revealed that spermatogenesis in <i>Jade1</i> cKO mice remained unaffected. <i>Jade1</i> cKO males also showed normal count, morphology, and fertility of the spermatozoa. Furthermore, deletion of JADE1 did not affect the dynamics of H4K16ac and protein levels of core histones (H2A, H2B, H3, and H4) during spermiogenesis in mice.</p> Conclusions <p>Based on these findings, we infer that JADE1 is dispensable for the dynamics of H4K16ac or core histone degradation during spermiogenesis, and is not essential for male fertility. Our findings provide new insights for further investigation of the biological functions of JADE1 in the murine model.</p>