Scholarlink

Background <p>Pancreatic cancer is known to be one of the most challenging and complex types of cancer owing to its late diagnosis, rapid growth, and resistance to treatment. The use of iron-nickel (Fe-Ni) nanoparticles in cancer treatment is an innovative method that can reduce side effects. This study investigates the anticancer effects of Fe-Ni nanoparticles on the activity of the PANC-1 cell line.</p> Methods <p>In this experimental study, after synthesizing Fe-Ni nanoparticles, their characterization was performed using UV-vis spectroscopy, TEM, and FE-SEM imaging. Subsequently, pancreatic cancer cells (PANC-1 cell line) and normal HUVEC were treated with these nanoparticles. Based on the obtained IC<sub>50</sub> values, cell viability was assessed using the MTT assay, and the apoptosis rate was analyzed using flow cytometry. Finally, the expression levels of the BAX and BCL-2 genes, as well as the microRNAs miR-212-3p and miR-221-3p, were evaluated using the quantitative real-time PCR method.</p> Result <p>The characterization results of the nanoparticles indicated that the Fe-Ni nanoparticles were within the optimal range for therapeutic applications in terms of shape and size. The IC50 for PANC-1 cells was 50.17&#xa0;µg/mL at 24&#xa0;h and 28.59&#xa0;µg/mL at 48&#xa0;h. In contrast, the IC50 for HUVEC cells exceeded 200&#xa0;µg/mL at both time points, indicating selective toxicity toward cancer cells. The findings revealed that the treatment of pancreatic cancer cells with Fe-Ni nanoparticles significantly reduced cell survival and induced apoptosis. Additionally, the nanoparticles modulated the genes involved in apoptosis and disrupted the expression of miR-221-3p and miR-212-3p, demonstrating further effects on this cell line.</p> Conclusion <p>Finally, the anticancer effects of Fe-Ni nanoparticles revealed that these nanoparticles can exhibit selective toxicity towards cancer cells and possess the ability to induce apoptosis.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluation of the effect of Fe–Ni nanoparticles on apoptosis through the modulation of miR-212-3p, miR-221-3p, BAX, and BCL-2 genes in pancreatic PANC-1 cells

  • 期刊论文

  • 发表日期：

    2025年10月30日

查看全文
  • Mahmood Oladi,
  • Mohammad Zaman,
  • Fatemeh Hajari Taheri,
  • Maryam Seyedolmohadesin

摘要

Background

Pancreatic cancer is known to be one of the most challenging and complex types of cancer owing to its late diagnosis, rapid growth, and resistance to treatment. The use of iron-nickel (Fe-Ni) nanoparticles in cancer treatment is an innovative method that can reduce side effects. This study investigates the anticancer effects of Fe-Ni nanoparticles on the activity of the PANC-1 cell line.

Methods

In this experimental study, after synthesizing Fe-Ni nanoparticles, their characterization was performed using UV-vis spectroscopy, TEM, and FE-SEM imaging. Subsequently, pancreatic cancer cells (PANC-1 cell line) and normal HUVEC were treated with these nanoparticles. Based on the obtained IC50 values, cell viability was assessed using the MTT assay, and the apoptosis rate was analyzed using flow cytometry. Finally, the expression levels of the BAX and BCL-2 genes, as well as the microRNAs miR-212-3p and miR-221-3p, were evaluated using the quantitative real-time PCR method.

Result

The characterization results of the nanoparticles indicated that the Fe-Ni nanoparticles were within the optimal range for therapeutic applications in terms of shape and size. The IC50 for PANC-1 cells was 50.17 µg/mL at 24 h and 28.59 µg/mL at 48 h. In contrast, the IC50 for HUVEC cells exceeded 200 µg/mL at both time points, indicating selective toxicity toward cancer cells. The findings revealed that the treatment of pancreatic cancer cells with Fe-Ni nanoparticles significantly reduced cell survival and induced apoptosis. Additionally, the nanoparticles modulated the genes involved in apoptosis and disrupted the expression of miR-221-3p and miR-212-3p, demonstrating further effects on this cell line.

Conclusion

Finally, the anticancer effects of Fe-Ni nanoparticles revealed that these nanoparticles can exhibit selective toxicity towards cancer cells and possess the ability to induce apoptosis.