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Background <p>Chronic obstructive pulmonary disease (COPD) is a common lung disease characterized by airway inflammation, with high morbidity and mortality, and an increasing frequency with age. Small, non-coding RNA molecules known as microRNAs (miRNAs) control the expression of genes following transcription. In COPD, miR-125a-5p and miR-126-3p regulate disease progression; miR-125a-5p is usually low, linked to inflammation, while miR-126-3p varies. This study aims to examine their expression in COPD patients.</p> Methods <p>The expression levels of miR-125a-5p and miR-126-3p were measured using real-time polymerase chain reaction (RT-PCR) in a total of 52 healthy individuals and 53 patients diagnosed with COPD, including 16 with stable COPD and 37 experiencing acute exacerbations. Expression data of miRNAs were calculated by log2 − delta delta CT (2<sup>−ΔΔCt</sup>). Since most variables did not meet the normality assumption (Shapiro–Wilk, <i>p</i> &lt; 0.05), non-parametric tests were applied. Specifically, the Mann–Whitney U test was used for pairwise comparisons (COPD vs. Control), while the Kruskal–Wallis test was used for comparisons among three groups (Acute, Stable, and Control). A p-value of &lt; 0.05 was considered statistically significant.</p> Results <p>The expression levels of miR-125a-5p were significantly lower and those of miR-126-3p significantly higher in COPD patients compared to controls (<i>p</i> = 0.045 and <i>p</i> = 0.034, respectively). However, when Acute COPD, Stable COPD, and Control groups were compared, no statistically significant differences were observed (<i>p</i> &gt; 0.05).</p> Conclusion <p>In this study, the expression level of miR-125a-5p was lower and miR-126-3p was higher in COPD patients compared to controls. However, no significant differences were observed among Acute, Stable, and Control groups.</p>

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Investigation of expression levels of MIR125A and MIR126 genes in chronic obstructive lung disease

  • 期刊论文

  • 发表日期：

    2025年10月23日

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  • Sumeyye Yildirim,
  • Nevin Karakus,
  • Gokhan Aykun

摘要

Background

Chronic obstructive pulmonary disease (COPD) is a common lung disease characterized by airway inflammation, with high morbidity and mortality, and an increasing frequency with age. Small, non-coding RNA molecules known as microRNAs (miRNAs) control the expression of genes following transcription. In COPD, miR-125a-5p and miR-126-3p regulate disease progression; miR-125a-5p is usually low, linked to inflammation, while miR-126-3p varies. This study aims to examine their expression in COPD patients.

Methods

The expression levels of miR-125a-5p and miR-126-3p were measured using real-time polymerase chain reaction (RT-PCR) in a total of 52 healthy individuals and 53 patients diagnosed with COPD, including 16 with stable COPD and 37 experiencing acute exacerbations. Expression data of miRNAs were calculated by log2 − delta delta CT (2−ΔΔCt). Since most variables did not meet the normality assumption (Shapiro–Wilk, p < 0.05), non-parametric tests were applied. Specifically, the Mann–Whitney U test was used for pairwise comparisons (COPD vs. Control), while the Kruskal–Wallis test was used for comparisons among three groups (Acute, Stable, and Control). A p-value of < 0.05 was considered statistically significant.

Results

The expression levels of miR-125a-5p were significantly lower and those of miR-126-3p significantly higher in COPD patients compared to controls (p = 0.045 and p = 0.034, respectively). However, when Acute COPD, Stable COPD, and Control groups were compared, no statistically significant differences were observed (p > 0.05).

Conclusion

In this study, the expression level of miR-125a-5p was lower and miR-126-3p was higher in COPD patients compared to controls. However, no significant differences were observed among Acute, Stable, and Control groups.