Scholarlink

Background and aim <p>The fatty acid (FA) composition of the gluten-free diet (GFD) may affect mucosal responses in celiac disease (CeD). This study evaluated the effects of oleic acid (OA) and palmitic acid (PA) on gliadin-induced inflammation and barrier dysfunction using Caco-2 cells.</p> Methods <p>Caco-2 cells were cultured under standard conditions and exposed to peptic–tryptic digested gliadin (PT-Gliadin) to induce a CeD-like response. Cells were treated with OA or PA under three conditions: co-treatment (with PT-Gliadin), pre-treatment (before PT-Gliadin), or post-treatment (after PT-Gliadin). Inflammatory gene expression (<i>NF-κB, TNF-α, IL-6, IL-15</i>) and tight junction markers (<i>ZO-1, occludin</i>) were quantified by qRT-PCR, while IL-6 and IL-15 secretion was measured by ELISA.</p> Results <p>PT-Gliadin significantly increased inflammatory gene expression and cytokine secretion, while reducing <i>ZO-1</i> and <i>occludin</i> levels (<i>p</i> &lt; 0.05). OA co-treatment significantly suppressed <i>NF-κB, TNF-α, IL-6,</i> and <i>IL-15</i> expression, lowered IL-6 and IL-15 secretion, and increased <i>ZO-1</i> and <i>occludin</i> levels (<i>p</i> &lt; 0.05). OA post-treatment also reduced <i>NF-κB</i> and <i>IL-15</i> expression (<i>p</i> &lt; 0.05) and diminished both IL-6 and IL-15 release (<i>p</i> &lt; 0.05). Conversely, PA co-treatment elevated <i>NF-κB, TNF-α, IL-6,</i> and <i>IL-15</i> expression (<i>p</i> &lt; 0.05), increased IL-6 secretion (<i>p</i> &lt; 0.05), and aggravated the loss of <i>ZO-1</i> and<i> occludin</i> (<i>p</i> &lt; 0.05). PA post-treatment similarly enhanced <i>NF-κB, TNF-α, IL-6,</i> and <i>IL-15</i> expression (<i>p</i> &lt; 0.05), increased IL-6 secretion (<i>p</i> &lt; 0.05), and reduced <i>occludin</i> level (<i>p</i> &lt; 0.05). Pre-treatment with either FA produced no significant effects.</p> Conclusion <p>OA mitigates, whereas PA exacerbates, gliadin-induced inflammation and barrier damage in Caco-2 cells. Modifying GFD fat composition to favor OA over PA may improve mucosal outcomes in CeD.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Evaluating the influence of oleic and palmitic acids on inflammatory markers and gut barrier integrity in a celiac disease Caco-2 model

  • 期刊论文

  • 发表日期：

    2025年9月22日

查看全文
  • Behrooz Ahmadi,
  • Abdolrahim Nikzamir,
  • Majid Sirati-Sabet,
  • Nastaran Asri,
  • Mohammad Rostami-Nejad

摘要

Background and aim

The fatty acid (FA) composition of the gluten-free diet (GFD) may affect mucosal responses in celiac disease (CeD). This study evaluated the effects of oleic acid (OA) and palmitic acid (PA) on gliadin-induced inflammation and barrier dysfunction using Caco-2 cells.

Methods

Caco-2 cells were cultured under standard conditions and exposed to peptic–tryptic digested gliadin (PT-Gliadin) to induce a CeD-like response. Cells were treated with OA or PA under three conditions: co-treatment (with PT-Gliadin), pre-treatment (before PT-Gliadin), or post-treatment (after PT-Gliadin). Inflammatory gene expression (NF-κB, TNF-α, IL-6, IL-15) and tight junction markers (ZO-1, occludin) were quantified by qRT-PCR, while IL-6 and IL-15 secretion was measured by ELISA.

Results

PT-Gliadin significantly increased inflammatory gene expression and cytokine secretion, while reducing ZO-1 and occludin levels (p < 0.05). OA co-treatment significantly suppressed NF-κB, TNF-α, IL-6, and IL-15 expression, lowered IL-6 and IL-15 secretion, and increased ZO-1 and occludin levels (p < 0.05). OA post-treatment also reduced NF-κB and IL-15 expression (p < 0.05) and diminished both IL-6 and IL-15 release (p < 0.05). Conversely, PA co-treatment elevated NF-κB, TNF-α, IL-6, and IL-15 expression (p < 0.05), increased IL-6 secretion (p < 0.05), and aggravated the loss of ZO-1 and occludin (p < 0.05). PA post-treatment similarly enhanced NF-κB, TNF-α, IL-6, and IL-15 expression (p < 0.05), increased IL-6 secretion (p < 0.05), and reduced occludin level (p < 0.05). Pre-treatment with either FA produced no significant effects.

Conclusion

OA mitigates, whereas PA exacerbates, gliadin-induced inflammation and barrier damage in Caco-2 cells. Modifying GFD fat composition to favor OA over PA may improve mucosal outcomes in CeD.