Scholarlink

Background <p>Chilli (<i>Capsicum annuum L.)</i> is a vegetable and spicy crop commonly grown in the tropical and subtropical regions. Chilli is affected by approximately seventy-five viruses, and the occurrence of severe losses is common because of the mixed infections of different viruses. An essential diagnostic process capable of simultaneously identifying several viruses is necessary for monitoring their transmission, implementing control strategies, and ensuring the use of virus-free plant materials.</p> Method <p>In this study, using the Rolling circle amplification (RCA) approach, we have identified three exemplar species of the begomovirus, <i>Begomovirus chillicapsici</i> [Chilli leaf curl virus (ChiLCV/Chi)], namely <i>Begomovirus capsibhavanisagarense</i> [chilli leaf curl Bhavanisagar virus (ChiLCBV)], chilli leaf curl Salem virus (ChiLCSV), and <i>Begomovirus capsisrilankaense</i> [chilli curl leaf Sri Lanka virus (ChiLCSLV)], from the infected chilli samples collected from Tamil Nadu, India. Three sets of species-specific primers were developed using the three clones to identify three distinct viruses in a single plant. We used multiplex PCR (mPCR), which included three sets of primers targeting distinct regions of the separate viruses. The mPCR test has a detection limit for specific concentration, allowing it to identify all target viruses in a single reaction. This indicated that the developed assay had appropriate sensitivity. This new test demonstrated excellent specificity and no cross-amplification.</p> Results <p>Multiplex PCR was verified using field samples obtained from different locations in Tamil Nadu, India. Out of 650 samples tested from the Tamil Nadu state of India, 65.8% showed single infections with ChiLCBV, 56.8% with ChiLCSV, and 55.2% with ChiLCSLV, while 59.2% had mixed infections involving all three viruses.</p> Conclusions <p>These findings indicate a high prevalence of both single and mixed infections in chilli crops. This study represents the first documentation of the development and field validation of an mPCR test for three chilli viruses. It is expected to be useful for regular viral indexing and management.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular characterization and multiplex PCR assay for the detection and identification of three different begomovirus species in the chilli fields of Tamil Nadu

  • 期刊论文

  • 发表日期：

    2025年9月20日

查看全文
  • Prayukta Padelkar,
  • P. Jayanthi,
  • A. Swapna Geetanjali

摘要

Background

Chilli (Capsicum annuum L.) is a vegetable and spicy crop commonly grown in the tropical and subtropical regions. Chilli is affected by approximately seventy-five viruses, and the occurrence of severe losses is common because of the mixed infections of different viruses. An essential diagnostic process capable of simultaneously identifying several viruses is necessary for monitoring their transmission, implementing control strategies, and ensuring the use of virus-free plant materials.

Method

In this study, using the Rolling circle amplification (RCA) approach, we have identified three exemplar species of the begomovirus, Begomovirus chillicapsici [Chilli leaf curl virus (ChiLCV/Chi)], namely Begomovirus capsibhavanisagarense [chilli leaf curl Bhavanisagar virus (ChiLCBV)], chilli leaf curl Salem virus (ChiLCSV), and Begomovirus capsisrilankaense [chilli curl leaf Sri Lanka virus (ChiLCSLV)], from the infected chilli samples collected from Tamil Nadu, India. Three sets of species-specific primers were developed using the three clones to identify three distinct viruses in a single plant. We used multiplex PCR (mPCR), which included three sets of primers targeting distinct regions of the separate viruses. The mPCR test has a detection limit for specific concentration, allowing it to identify all target viruses in a single reaction. This indicated that the developed assay had appropriate sensitivity. This new test demonstrated excellent specificity and no cross-amplification.

Results

Multiplex PCR was verified using field samples obtained from different locations in Tamil Nadu, India. Out of 650 samples tested from the Tamil Nadu state of India, 65.8% showed single infections with ChiLCBV, 56.8% with ChiLCSV, and 55.2% with ChiLCSLV, while 59.2% had mixed infections involving all three viruses.

Conclusions

These findings indicate a high prevalence of both single and mixed infections in chilli crops. This study represents the first documentation of the development and field validation of an mPCR test for three chilli viruses. It is expected to be useful for regular viral indexing and management.