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Background <p><i>Centella asiatica</i> L. plays a significant role in the traditional medicine of the Indigenous Mizo communities, valued for its healing abilities, especially in addressing wounds, skin ailments, and gastrointestinal issues.</p> Methods and results <p>This study aims to isolate a potential endophytic actinobacteria from <i>Centella asiatica</i> L. with antimicrobial and antioxidant properties. Initially, five endophytic actinobacterial strains were isolated from <i>Centella asiatica</i> using ISP7 as a growth medium. CENK12 was the most promising isolate, inhibiting 11 out of 15 clinical multidrug-resistant <i>Staphylococcus aureus</i> (MDRSA) pathogens using agar well diffusion method. Scanning electron microscopy (SEM) revealed its morphology and 16&#xa0;S rRNA sequencing identified it as <i>Streptomyces sp.</i> strain CENK12. Phytochemicals such as phenols and flavonoids, which have been reported in the host plant <i>Centella asiatica</i>, were also quantified in the endophytic Streptomyces sp. The results showed the presence of both phenols and flavonoids. The total phenolic content was 68.6 ± 0.51&#xa0;mg of Gallic Acid Equivalent (GAE) for every gram of extract, and the total flavonoid content was 10.92 ± 0.64&#xa0;mg of Quercetin (QE) for every gram of the extract. Antioxidant activity, assessed using ABTS method and DPPH assay and revealed an antioxidant capacity of IC<sub>50</sub> = 82.78&#xa0;µg/ml and IC<sub>50</sub> = 668.3 ± 0.92&#xa0;µg/mL for ABTS and DPPH assay respectively. GC-MS analysis detected 20 volatile organic compounds. Additionally, chromatographic analysis of the methanol fraction was conducted using high-performance thin-layer chromatography (HPTLC) with two optimized solvent systems designed for glycosides and phenols: Ethyl acetate, methanol, and water in a ratio of 20:3:2, and cyclohexane, ethyl acetate, and formic acid in a ratio of 4:6:1.</p> Conclusion <p>This study provides a strong evidence that <i>Streptomyces</i> sp. (CENK12) is a promising agent for the development of new drug that could fight multidrug-resistant <i>Staphylococcus aureus</i> (MDRSA) and moreover its potent antioxidant activity suggests its potential as a novel antioxidants agent.</p>

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Streptomyces sp. strain CENK12 from Centella asiatica L.: a potential source of antimicrobial and antioxidant agents against multidrug-resistant Staphylococcus aureus

  • 期刊论文

  • 发表日期：

    2025年8月26日

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  • Lalrokimi,
  • Awmpuizeli Fanai,
  • Felicia Lalremruati,
  • Beirachhitha Bohia,
  • Nancy Lalhriatpuii,
  • Lalrosangpuii,
  • C. Lalmuansangi,
  • Zothanpuia,
  • Esther Lalnunmawii

摘要

Background

Centella asiatica L. plays a significant role in the traditional medicine of the Indigenous Mizo communities, valued for its healing abilities, especially in addressing wounds, skin ailments, and gastrointestinal issues.

Methods and results

This study aims to isolate a potential endophytic actinobacteria from Centella asiatica L. with antimicrobial and antioxidant properties. Initially, five endophytic actinobacterial strains were isolated from Centella asiatica using ISP7 as a growth medium. CENK12 was the most promising isolate, inhibiting 11 out of 15 clinical multidrug-resistant Staphylococcus aureus (MDRSA) pathogens using agar well diffusion method. Scanning electron microscopy (SEM) revealed its morphology and 16 S rRNA sequencing identified it as Streptomyces sp. strain CENK12. Phytochemicals such as phenols and flavonoids, which have been reported in the host plant Centella asiatica, were also quantified in the endophytic Streptomyces sp. The results showed the presence of both phenols and flavonoids. The total phenolic content was 68.6 ± 0.51 mg of Gallic Acid Equivalent (GAE) for every gram of extract, and the total flavonoid content was 10.92 ± 0.64 mg of Quercetin (QE) for every gram of the extract. Antioxidant activity, assessed using ABTS method and DPPH assay and revealed an antioxidant capacity of IC50 = 82.78 µg/ml and IC50 = 668.3 ± 0.92 µg/mL for ABTS and DPPH assay respectively. GC-MS analysis detected 20 volatile organic compounds. Additionally, chromatographic analysis of the methanol fraction was conducted using high-performance thin-layer chromatography (HPTLC) with two optimized solvent systems designed for glycosides and phenols: Ethyl acetate, methanol, and water in a ratio of 20:3:2, and cyclohexane, ethyl acetate, and formic acid in a ratio of 4:6:1.

Conclusion

This study provides a strong evidence that Streptomyces sp. (CENK12) is a promising agent for the development of new drug that could fight multidrug-resistant Staphylococcus aureus (MDRSA) and moreover its potent antioxidant activity suggests its potential as a novel antioxidants agent.