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Background <p>Begomoviruses pose the deadliest threat to the productivity of most field crops especially vegetables like tomatoes. Mixed infections of begomoviruses under natural conditions often promote the frequent evolution of new species through genetic recombination between their genomes. <i>Ty</i>-genes of tomato are reported to provide resistance/tolerance to these begomoviruses.</p> Methods and results <p>In this study, PCR-based screening of 17 tomato genotypes with different <i>Ty-</i>gene combinations, along with a susceptible check cv. Pusa Ruby showed the presence of a weed-infecting begomovirus, croton yellow vein mosaic virus (CYVMV) in most of the (13 out of 18) genotypes tested. The complete genome of the virus was cloned and sequenced, revealing a maximum sequence identity of 90.6% with a different begomovirus species croton yellow vein leaf curl virus (CYVLCV). However, analysis of individual open reading frame (ORF) showed that ORF C1, C4, and the left intergenic region of the virus showed maximum sequence identity with tobacco curly shoot virus (TbCSV). ORF V1, V2, C2, and the right intergenic region showed maximum sequence identity with CYVMV. Phylogenetic profiling followed by recombination analysis showed the presence of recombination breakpoints in the viral genome.</p> Conclusion <p>Results indicated that the present virus may have evolved through interspecific recombination between CYVMV and TbCSV. The name tomato leaf curl Ty Pusa virus (ToLCTyPV) was proposed for the newly identified virus. Hence, the present study shows genetic recombination as the driving force for the evolution of a new begomovirus associated with leaf curl disease in <i>Ty</i>-resistance gene-containing tomato genotypes.</p>

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A novel interspecific-recombinant monopartite begomovirus emerging in tomato (Solanum lycopersicum L.) genotypes with Ty-resistant genes

  • 期刊论文

  • 发表日期：

    2025年6月12日

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  • Firoz Mondal,
  • Shipra Saxena,
  • Zakir Hussain,
  • Bikash Mandal,
  • Anirban Roy

摘要

Background

Begomoviruses pose the deadliest threat to the productivity of most field crops especially vegetables like tomatoes. Mixed infections of begomoviruses under natural conditions often promote the frequent evolution of new species through genetic recombination between their genomes. Ty-genes of tomato are reported to provide resistance/tolerance to these begomoviruses.

Methods and results

In this study, PCR-based screening of 17 tomato genotypes with different Ty-gene combinations, along with a susceptible check cv. Pusa Ruby showed the presence of a weed-infecting begomovirus, croton yellow vein mosaic virus (CYVMV) in most of the (13 out of 18) genotypes tested. The complete genome of the virus was cloned and sequenced, revealing a maximum sequence identity of 90.6% with a different begomovirus species croton yellow vein leaf curl virus (CYVLCV). However, analysis of individual open reading frame (ORF) showed that ORF C1, C4, and the left intergenic region of the virus showed maximum sequence identity with tobacco curly shoot virus (TbCSV). ORF V1, V2, C2, and the right intergenic region showed maximum sequence identity with CYVMV. Phylogenetic profiling followed by recombination analysis showed the presence of recombination breakpoints in the viral genome.

Conclusion

Results indicated that the present virus may have evolved through interspecific recombination between CYVMV and TbCSV. The name tomato leaf curl Ty Pusa virus (ToLCTyPV) was proposed for the newly identified virus. Hence, the present study shows genetic recombination as the driving force for the evolution of a new begomovirus associated with leaf curl disease in Ty-resistance gene-containing tomato genotypes.