Scholarlink

Background <p>Tomato leaf curl viral disease (ToLCV) is a major biotic stress, threatening tomato cultivation. However, limited information exists on the prevalence and diversity of ToLCV in Tamil Nadu, India and hence this study focused on identifying and characterizing such begomoviruses.</p> Methods and results <p>Tomato samples with infected symptoms were collected from major tomato-growing tracts of Tamil Nadu during 2023 to 2024 and subjected to polymerase chain reaction (PCR) using degenerate primers specific to ToLCV. Further, by employing rolling circle amplification (RCA), full-length sequences of viral genomes (∼2.7&#xa0;kb), alphasatellites and betasatellites (∼1.35&#xa0;kb each) were also obtained for three samples collected from different locations of Tamil Nadu (Dharmapuri (DPI-03), Krishnagiri (KGI-01) and Salem (SLM-01)). Diverse begomoviruses, including tomato leaf curl New Delhi virus (ToLCNDV), tomato leaf curl Palampur virus (ToLCPalV), tomato leaf curl Bangalore virus (ToLCBaV), and tomato leaf curl Gujarat virus (ToLCGUV) were identified in these three samples. Additionally, an alphasatellite (cotton leaf curl Multan alphasatellite, CLCuMuA), and two betasatellites (tomato leaf curl Bangalore betasatellite, ToLCBaB and tomato leaf curl Gujarat betasatellite, ToLCGUB) were also identified in KGI-01 and SLM-01. Evaluation of phylogenetic relationship and genetic variability revealed distinct clustering of begomoviruses and satellite sequences with high nucleotide diversity. In addition, recombinant analysis predicted inter- and intra- species recombination events.</p> Conclusion <p>This study identified the co-occurrence of ToLCNDV with ToLCPalV, ToLCBaV with CLCuMuA and ToLCBaB, and ToLCGUV with ToLCGUB within the same plants. These findings underscore the diverse nature of the begomovirus-satellite complex infecting tomatoes in Tamil Nadu and emphasize the urgent need to develop effective plant protection strategies.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Recombination, phylogenetic insights and co-existence dynamics of begomoviruses and satellite DNAs: implications for tomato viral disease management

  • 期刊论文

  • 发表日期：

    2025年4月15日

查看全文
  • Murugesan Naveen,
  • Pagalahalli Sankaran Shanmugam,
  • Ramasamy Srinivasan,
  • Mookiah Shanthi,
  • Marimuthu Murugan,
  • Kathiresan Sivagnanapazham,
  • Kathithachalam Angappan,
  • Ricardo Oliva,
  • Gandhi Karthikeyan,
  • Narayanan Manikanda Boopathi

摘要

Background

Tomato leaf curl viral disease (ToLCV) is a major biotic stress, threatening tomato cultivation. However, limited information exists on the prevalence and diversity of ToLCV in Tamil Nadu, India and hence this study focused on identifying and characterizing such begomoviruses.

Methods and results

Tomato samples with infected symptoms were collected from major tomato-growing tracts of Tamil Nadu during 2023 to 2024 and subjected to polymerase chain reaction (PCR) using degenerate primers specific to ToLCV. Further, by employing rolling circle amplification (RCA), full-length sequences of viral genomes (∼2.7 kb), alphasatellites and betasatellites (∼1.35 kb each) were also obtained for three samples collected from different locations of Tamil Nadu (Dharmapuri (DPI-03), Krishnagiri (KGI-01) and Salem (SLM-01)). Diverse begomoviruses, including tomato leaf curl New Delhi virus (ToLCNDV), tomato leaf curl Palampur virus (ToLCPalV), tomato leaf curl Bangalore virus (ToLCBaV), and tomato leaf curl Gujarat virus (ToLCGUV) were identified in these three samples. Additionally, an alphasatellite (cotton leaf curl Multan alphasatellite, CLCuMuA), and two betasatellites (tomato leaf curl Bangalore betasatellite, ToLCBaB and tomato leaf curl Gujarat betasatellite, ToLCGUB) were also identified in KGI-01 and SLM-01. Evaluation of phylogenetic relationship and genetic variability revealed distinct clustering of begomoviruses and satellite sequences with high nucleotide diversity. In addition, recombinant analysis predicted inter- and intra- species recombination events.

Conclusion

This study identified the co-occurrence of ToLCNDV with ToLCPalV, ToLCBaV with CLCuMuA and ToLCBaB, and ToLCGUV with ToLCGUB within the same plants. These findings underscore the diverse nature of the begomovirus-satellite complex infecting tomatoes in Tamil Nadu and emphasize the urgent need to develop effective plant protection strategies.