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Introduction <p>Hepatitis C virus (HCV) causes hepatitis worldwide. Detection of HCV infection is important for diagnosis and treatment. The most frequently used method for diagnosis is the detection of the 5’ untranslated (5’ UTR) conserved target using polymerase chain reaction (PCR). Unfortunately, the detection of the 5’UTR is not completely reliable. The 5’UTR may not be the best target for nucleic acid detection because of its complex secondary structures and the variations of different HCV strains. An alternative target is the 3’UTR. The purpose is to evaluate the diagnostic potential of the 3’UTR of HCV.</p> Methods and results <p>RNA was extracted from sixty-three serum samples. The 3’UTR and 5’UTR were amplified. Within the fifty-nine anti-HCV-positive samples, targeting the HCV 3’UTR and 5’UTR resulted in amplification in 40 (67.8%) and 54 (91.5%) of the samples, respectively. Five samples that were not amplified by 5’UTR detection were found positive by 3’UTR detection.</p> Conclusion <p>These results indicate that PCR detection of the HCV 3’UTR appears less effective than detection of the 5’UTR. Nevertheless, the detection of several regions of the HCV genome by multiplex PCR may improve the accuracy of HCV detection.</p>

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A diagnostic target in the 3’ untranslated region of hepatitis C virus genome

  • 期刊论文

  • 发表日期：

    2025年4月7日

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  • Weenassarin Ampoot,
  • Suthipong Chujan,
  • Anusorn Kiattanaphon,
  • Anchalee Sistayanarain

摘要

Introduction

Hepatitis C virus (HCV) causes hepatitis worldwide. Detection of HCV infection is important for diagnosis and treatment. The most frequently used method for diagnosis is the detection of the 5’ untranslated (5’ UTR) conserved target using polymerase chain reaction (PCR). Unfortunately, the detection of the 5’UTR is not completely reliable. The 5’UTR may not be the best target for nucleic acid detection because of its complex secondary structures and the variations of different HCV strains. An alternative target is the 3’UTR. The purpose is to evaluate the diagnostic potential of the 3’UTR of HCV.

Methods and results

RNA was extracted from sixty-three serum samples. The 3’UTR and 5’UTR were amplified. Within the fifty-nine anti-HCV-positive samples, targeting the HCV 3’UTR and 5’UTR resulted in amplification in 40 (67.8%) and 54 (91.5%) of the samples, respectively. Five samples that were not amplified by 5’UTR detection were found positive by 3’UTR detection.

Conclusion

These results indicate that PCR detection of the HCV 3’UTR appears less effective than detection of the 5’UTR. Nevertheless, the detection of several regions of the HCV genome by multiplex PCR may improve the accuracy of HCV detection.