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Background <p><i>Proteus mirabilis</i> (<i>P. mirabilis</i>), a Gram-negative bacterium from the <i>Morganellaceae</i> family, exists in various habitats, ranging from natural environmental sources to animal and human host. Two-component systems (TCSs) are involved in sensing various types of stimuli present in the environment, including antimicrobial chemotherapeutics. Moreover, TCSs regulate bacterial virulence. The 16 sets of genes encoding TCSs proteins have been identified in genome of <i>P. mirabilis</i> HI4320 reference strain, but their role remains understudied.</p> Methods and results <p>In the presented work, a comparative in silico analysis of TCSs proteins encoded by the genome sequences of <i>P. mirabilis</i> strains obtained from public databases was performed. Additionally, the expression of genes encoding regulatory proteins in response to polymyxin B and H<sub>2</sub>O<sub>2</sub> was analyzed using RT-qPCR. The obtained results revealed a relative conservatism of TCSs among <i>P. mirabilis</i> isolates, indicating, however, differences that might result in strains phenotypic diversity. Moreover, constitutive expression of genes coding regulatory proteins from studied TCSs was observed under laboratory conditions.</p> Conclusions <p>Presented study shed light on the role of TCSs in <i>P. mirabilis</i>. Further deepen research in this area might provide more effective means for controlling <i>P. mirabilis</i> infections.</p>

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In silico investigation and expression analysis of two-component regulatory systems in Proteus mirabilis

  • 期刊论文

  • 发表日期：

    2025年2月4日

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  • Dawid Gmiter,
  • Aleksandra Omelaniuk,
  • Wanesa Sasal,
  • Leon Petruńko,
  • Klaudia Musiał,
  • Sylwia Nawrot,
  • Ilona Pacak,
  • Wiesław Kaca

摘要

Background

Proteus mirabilis (P. mirabilis), a Gram-negative bacterium from the Morganellaceae family, exists in various habitats, ranging from natural environmental sources to animal and human host. Two-component systems (TCSs) are involved in sensing various types of stimuli present in the environment, including antimicrobial chemotherapeutics. Moreover, TCSs regulate bacterial virulence. The 16 sets of genes encoding TCSs proteins have been identified in genome of P. mirabilis HI4320 reference strain, but their role remains understudied.

Methods and results

In the presented work, a comparative in silico analysis of TCSs proteins encoded by the genome sequences of P. mirabilis strains obtained from public databases was performed. Additionally, the expression of genes encoding regulatory proteins in response to polymyxin B and H2O2 was analyzed using RT-qPCR. The obtained results revealed a relative conservatism of TCSs among P. mirabilis isolates, indicating, however, differences that might result in strains phenotypic diversity. Moreover, constitutive expression of genes coding regulatory proteins from studied TCSs was observed under laboratory conditions.

Conclusions

Presented study shed light on the role of TCSs in P. mirabilis. Further deepen research in this area might provide more effective means for controlling P. mirabilis infections.