Evaluation of human papillomavirus type 16 viral load and genome physical status in Iranian women with cervical disease

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发表日期：

2024年3月11日

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Shadi Setayeshi,
Ali Hasanzadeh,
Yousef Yahyapour,
Ahad Alizadeh,
Hossein Ghorbani,
Fahimeh Nokhostin,
Meghdad Bagheri,
Farzin Sadeghi

摘要

Background

This study examined the viral load and physical status of the human papillomavirus 16 (HPV-16) genome in non-cancerous, precancerous and cancerous cervical lesions.

Methods

Quantitative real-time PCR was performed to determine HPV-16 E2 and E6 viral load in 132 cervical specimens. E2/E6 viral load ratio was used to determine the physical status of HPV-16 genome.

Results

E2 gene viral load was a significant (P < 0.001) predicting biomarker in differentiating non-cancerous from precancerous and cancerous samples. E6 gene viral load was significantly different between the groups (P < 0.001). The specificity and sensitivity of E2 and E6 in distinguishing SCC samples were 100% and 95% respectively.

Conclusion

HPV-16 viral load measured through E2 and E6 genes is a reliable indicator of lesion type.