Maternal factor Trim75 contributes to zygotic genome activation program in mouse early embryos

期刊论文
发表日期：

2024年4月20日

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Weibo Hou,
Lijun Chen,
Jingzhang Ji,
Songling Xiao,
Hongye Linghu,
Lixin Zhang,
Yue Ping,
Chunsheng Wang,
Qingran Kong,
Wenpin Cai,
Xu Yang

摘要

Background

Zygotic genome activation (ZGA) is an important event in the early embryo development, and human embryo developmental arrest has been highly correlated with ZGA failure in clinical studies. Although a few studies have linked maternal factors to mammalian ZGA, more studies are needed to fully elucidate the maternal factors that are involved in ZGA.

Methods and results

In this study, we utilized published single-cell RNA sequencing data from a Dux-mediated mouse embryonic stem cell to induce a 2-cell-like transition state and selected potential drivers for the transition according to an RNA velocity analysis.

Conclusions

An overlap of potential candidate markers of 2-cell-like-cells identified in this research with markers generated by various data sets suggests that Trim75 is a potential driver of minor ZGA and may recruit EP300 and establish H3K27ac in the gene body of minor ZGA genes, thereby contributing to mammalian preimplantation embryo development.