Differential DNA methylation and CTCF binding between the ESR1 promoter a of MCF-7 and MDA-MB-231 breast cancer cells

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2024年1月18日

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Edén Víctor Montes-de-Oca-Fuentes,
Karina Jácome-López,
Anaís Zarco-Mendoza,
Georgina Guerrero,
José Luis Ventura-Gallegos,
Sergio Juárez-Méndez,
Alberto Jose Cabrera-Quintero,
Félix Recillas-Targa,
Alejandro Zentella-Dehesa

摘要

Background

ESR1 is expressed by 60–70% of breast tumours. it’s a good prognosis factor and the target of hormone therapy. Optimization of ESR1 reactivation therapy is currently ongoing. Here we probe if the transcription factor CTCF plays a role in the differential expression of ESR1 in the breast cancer cell lines MCF-7 (ESR1+) and MDA-MB-231 (ESR1-).

Methods and results

Knockdown of CTCF in MCF-7 resulted in decreased ESR1 gene expression. CTCF binds to the promoter of ESR1 in MCF-7 but not in MDA-MB-231 cells. CTCF ESR1 binding sites are unmethylated in MCF7 but methylated in MDA-MB-231 cells.

Conclusion

ESR1 expression in MCF7 cells is dependent on CTCF expression. CTCF can bind to specific regions of the promotor of ESR1 gene in MCF-7 cells but not in MDA-MB-231 cells, this correlates with the methylation status of these regions and could be involved in the transcriptional regulation of ESR1.