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<p>Tiller number is a pivotal agronomic trait governing wheat yield potential, yet its underlying genetic regulation remains insufficiently elucidated. Here, we report the map-based cloning of <i>tin7</i>, a major gene controlling tiller number in wheat. Using near-isogenic lines (NIL-H, high-tillering; NIL-L, low-tillering), we fine-mapped <i>tin7</i>. Eleven polymorphic markers genotyped 10,753&#xa0;F₂ individuals, <i>tin7</i> was mapped to a 400.61&#xa0;kb physical interval in Chinese Spring genome, which contained 16 high-confidence genes in Chinese Spring (17 high-confidence genes in emmer wheat). Sequence and expression analyses identified <i>TraesCS2B01G619900</i> (ortholog of emmer wheat <i>TRIDC2BG090810</i>) as the candidate for <i>tin7</i>, with NIL-L harboring a premature stop codon. Functional validation via EMS-induced and CRISPR/Cas9-mediated mutants confirmed its role: EMS mutants showed 67.90-80.25% tiller reduction, while CRISPR/Cas9 mutants in KN199 exhibited a significant reduction (37.31–62.88%). These findings confirm <i>TraesCS2B01G619900</i> is <i>tin7</i>, regulating wheat tillering and providing a valuable genetic resource for yield improvement via molecular breeding.</p>

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Fine mapping and verification of a tiller inhibition gene in synthetic hexaploid wheat derivative

  • 期刊论文

  • 发表日期：

    2026年2月7日

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  • Shuai Hou,
  • Lang Yu,
  • Wenjie Guo,
  • Yuzhou Mou,
  • Haojie Li,
  • Fengcheng Shi,
  • Jian Zhou,
  • Zhiqiang Wang,
  • Yu Lin,
  • Yaxi Liu

摘要

Tiller number is a pivotal agronomic trait governing wheat yield potential, yet its underlying genetic regulation remains insufficiently elucidated. Here, we report the map-based cloning of tin7, a major gene controlling tiller number in wheat. Using near-isogenic lines (NIL-H, high-tillering; NIL-L, low-tillering), we fine-mapped tin7. Eleven polymorphic markers genotyped 10,753 F₂ individuals, tin7 was mapped to a 400.61 kb physical interval in Chinese Spring genome, which contained 16 high-confidence genes in Chinese Spring (17 high-confidence genes in emmer wheat). Sequence and expression analyses identified TraesCS2B01G619900 (ortholog of emmer wheat TRIDC2BG090810) as the candidate for tin7, with NIL-L harboring a premature stop codon. Functional validation via EMS-induced and CRISPR/Cas9-mediated mutants confirmed its role: EMS mutants showed 67.90-80.25% tiller reduction, while CRISPR/Cas9 mutants in KN199 exhibited a significant reduction (37.31–62.88%). These findings confirm TraesCS2B01G619900 is tin7, regulating wheat tillering and providing a valuable genetic resource for yield improvement via molecular breeding.