Scholarlink

<p>Rice black-streaked dwarf virus (RBSDV) disease, caused by rice black-streaked dwarf virus and transmitted by the small brown planthopper (<i>Laodelphax striatellus</i> Fallén, SBPH), is a devastating viral disease severely impacting global rice production. The cultivation and promotion of resistant varieties is considered the most economical and effective control strategy. However, effectiveness of field-based resistance identification is hindered by environmental variability, seasonal limitations of SBPH outbreaks, and inconsistent virus pressure, leading to unreliable results. Therefore, we developed an efficient and reliable artificial inoculation system for RBSDV-resistant rice materials screening under controllable laboratory conditions. This system encompasses optimized procedures for material preparation, inoculation methodology, disease phenotyping and resistance evaluation. We directly inoculated 1st-2nd instar SBPH nymphs onto the RBSDV infected plants and obtained nymphs with high viruliferous rates. Then, to generate a stable laboratory virus source, these viruliferous nymphs were used to inoculate highly susceptible rice seedlings (e.g., Suyunuo). Subsequent inoculation cycles using these viruliferous rice seedlings consistently produced SBPH populations with high viruliferous rates (average about 42.4%). This system enables continuous, year-round resistance evaluation, breaking through the limitation of environmental dependence in field identification. This method will significantly accelerate the breeding of RBSDV disease resistant rice varieties.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

An artificial inoculation method for evaluating rice resistance to RBSDV

  • 期刊论文

  • 发表日期：

    2025年10月23日

查看全文
  • Quanguang Sun,
  • Hongliang Zhang,
  • Jun He,
  • Qi Li,
  • Ling Jiang,
  • Yunlong Wang,
  • Yongsheng Wang,
  • Yuqiang Liu,
  • Jianmin Wan

摘要

Rice black-streaked dwarf virus (RBSDV) disease, caused by rice black-streaked dwarf virus and transmitted by the small brown planthopper (Laodelphax striatellus Fallén, SBPH), is a devastating viral disease severely impacting global rice production. The cultivation and promotion of resistant varieties is considered the most economical and effective control strategy. However, effectiveness of field-based resistance identification is hindered by environmental variability, seasonal limitations of SBPH outbreaks, and inconsistent virus pressure, leading to unreliable results. Therefore, we developed an efficient and reliable artificial inoculation system for RBSDV-resistant rice materials screening under controllable laboratory conditions. This system encompasses optimized procedures for material preparation, inoculation methodology, disease phenotyping and resistance evaluation. We directly inoculated 1st-2nd instar SBPH nymphs onto the RBSDV infected plants and obtained nymphs with high viruliferous rates. Then, to generate a stable laboratory virus source, these viruliferous nymphs were used to inoculate highly susceptible rice seedlings (e.g., Suyunuo). Subsequent inoculation cycles using these viruliferous rice seedlings consistently produced SBPH populations with high viruliferous rates (average about 42.4%). This system enables continuous, year-round resistance evaluation, breaking through the limitation of environmental dependence in field identification. This method will significantly accelerate the breeding of RBSDV disease resistant rice varieties.