Scholarlink

<p>Snap bean (<i>Phaseolus vulgaris</i> L.), a nutrient-rich crop, is limited by <i>Bean common mosaic virus</i> (BCMV) and <i>Bean common mosaic necrosis virus</i> (BCMNV). To better understand the genetic basis of resistance, 376 snap bean accessions and 12&#xa0;F₂ populations were screened with US-6 [pathogroup (PG)-VII], NL-8 (PG-III), or NL-3 (PG-VI) strains and genotyped for <i>I</i>, <i>bc-1</i>, and <i>bc-3</i> loci using existing candidate gene-linked markers. Two Vps4 candidate genes associated with <i>bc-u</i> and <i>bc-2</i> recessive loci were re-sequenced to identify novel mutations. Two major allelic groups of <i>bc-u</i> missense mutations were identified: (<i>bc-u</i><sup>a</sup> = <i>bc-u</i><sup>d</sup> = <i>bc-u</i><sup>g</sup>) and (<i>bc-u</i><sup>r</sup> ≤ <i>bc-u</i><sup>s</sup>), each interacting differently with <i>bc-1</i> and <i>bc-2</i> to confer strain-specific resistance or susceptibility to NL-3 and US-6. New <i>bc-2</i><sup>[IVT 7214]</sup> and existing <i>bc-2</i><sup>[Robust]</sup> and <i>bc-2</i><sup>[UI−111]</sup> nonsense mutations exhibited functionally equivalent alleles, resulting in the same genetic effect across pathogroups. Twenty-six allelic combinations were found, 16 of which included the dominant <i>I</i> gene. Overall, 336 of 376 accessions carried <i>I</i>, of which 233 possessed <i>I</i> + <i>RNase H-Like 1</i><sup>C</sup> + <i>bc-1</i> combination characteristic of host group (HG)-9 cultivars with restricted vein necrosis (VN) to NL-8. Importantly, 30 accessions possessed <i>I</i> + <i>RNase H-Like 1</i><sup>C</sup> + <i>bc-1</i> + <i>bc-u</i><sup>a, d, or g</sup>, conferring VN to NL-3 strain, characteristic of HG-10 cultivars. This panel provides a valuable resource of resistance diversity to BCMV and BCMNV, which can be used in breeding programs with the markers described. The <i>bc-2</i> and <i>bc-3</i> alleles, found in only one or two accessions, offer untapped resistance sources for snap bean.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Snap bean diversity for resistance to bean common mosaic and necrosis viruses examined using new and existing candidate gene-linked markers

  • 期刊论文

  • 发表日期：

    2025年11月27日

查看全文
  • Alvaro Soler-Garzón,
  • John P. Hart,
  • Phillip N. Miklas

摘要

Snap bean (Phaseolus vulgaris L.), a nutrient-rich crop, is limited by Bean common mosaic virus (BCMV) and Bean common mosaic necrosis virus (BCMNV). To better understand the genetic basis of resistance, 376 snap bean accessions and 12 F₂ populations were screened with US-6 [pathogroup (PG)-VII], NL-8 (PG-III), or NL-3 (PG-VI) strains and genotyped for I, bc-1, and bc-3 loci using existing candidate gene-linked markers. Two Vps4 candidate genes associated with bc-u and bc-2 recessive loci were re-sequenced to identify novel mutations. Two major allelic groups of bc-u missense mutations were identified: (bc-ua = bc-ud = bc-ug) and (bc-ur ≤ bc-us), each interacting differently with bc-1 and bc-2 to confer strain-specific resistance or susceptibility to NL-3 and US-6. New bc-2[IVT 7214] and existing bc-2[Robust] and bc-2[UI−111] nonsense mutations exhibited functionally equivalent alleles, resulting in the same genetic effect across pathogroups. Twenty-six allelic combinations were found, 16 of which included the dominant I gene. Overall, 336 of 376 accessions carried I, of which 233 possessed I + RNase H-Like 1C + bc-1 combination characteristic of host group (HG)-9 cultivars with restricted vein necrosis (VN) to NL-8. Importantly, 30 accessions possessed I + RNase H-Like 1C + bc-1 + bc-ua, d, or g, conferring VN to NL-3 strain, characteristic of HG-10 cultivars. This panel provides a valuable resource of resistance diversity to BCMV and BCMNV, which can be used in breeding programs with the markers described. The bc-2 and bc-3 alleles, found in only one or two accessions, offer untapped resistance sources for snap bean.